Academic Journal
Learning about Fluorescence in Undergraduate Biochemistry: Enzyme Kinetics Using a Low-Cost, Student-Built Fluorescence Spectrometer
| العنوان: | Learning about Fluorescence in Undergraduate Biochemistry: Enzyme Kinetics Using a Low-Cost, Student-Built Fluorescence Spectrometer |
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| اللغة: | English |
| المؤلفون: | Keithley, Richard B., Sullivan, Dylan T., Dodd, John M., II, Iyer, Kavya V., Sarisky, Catherine A. (ORCID |
| المصدر: | Journal of Chemical Education. Dec 2021 98(12):4054-4060. |
| الاتاحة: | Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc |
| Peer Reviewed: | Y |
| Page Count: | 7 |
| تاريخ النشر: | 2021 |
| نوع الوثيقة: | Journal Articles Reports - Descriptive |
| Education Level: | Higher Education Postsecondary Education |
| Descriptors: | Chemistry, Science Instruction, College Science, Undergraduate Study, Spectroscopy, Biochemistry, Science Laboratories, Laboratory Equipment, Scientific Concepts, Light |
| DOI: | 10.1021/acs.jchemed.1c00912 |
| تدمد: | 0021-9584 |
| مستخلص: | Techniques and instrumentation that involve fluorescence are used in many careers related to biology and biochemistry. However, due to the expense of instrumentation, the theory and practice of fluorescence spectroscopy is commonly concentrated in instrumental analysis in most curricula, a class rarely required for biology and biochemistry majors. Biochemistry represents a course taken by these majors, providing an opportunity to expose them to the fundamentals of fluorescence. A three session laboratory series has been developed where the kinetic analysis of lactate dehydrogenase is carried out through the use of inexpensive, student-built fluorometers. These instruments are optimized for the detection of NADH fluorescence and constructed with interlocking building blocks (such as LEGO blocks), an LED source, a phototransistor detector, and other commercially available materials for a total cost of $52. Biochemistry students have built these spectrometers and used them to determine the K[subscript m] of "Gallus gallus" lactate dehydrogenase for L-lactate and the k[subscript cat] of this enzyme in accordance with literature values. Learning outcomes were assessed and indicate proficient understanding of instrument use and design. |
| Abstractor: | As Provided |
| Entry Date: | 2021 |
| رقم الانضمام: | EJ1319523 |
| قاعدة البيانات: | ERIC |
| تدمد: | 0021-9584 |
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| DOI: | 10.1021/acs.jchemed.1c00912 |