Supported lipid bilayer repair mediated by AH peptide
| العنوان: | Supported lipid bilayer repair mediated by AH peptide |
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| المؤلفون: | Kim, M. C., Gunnarsson, Anders, 1981, Tabaei, Seyed, 1978, Höök, Fredrik, 1966, Cho, N. J. |
| المصدر: | Physical Chemistry Chemical Physics. 18(4):3040-3047 |
| مصطلحات موضوعية: | in-situ, alpha-helical peptide, membranes, rupture, quartz-crystal microbalance, unilamellar vesicles, dissipation, vesicle adsorption, phospholipid-bilayers, osmotic-pressure |
| الوصف: | The adsorption and fusion of small unilamellar lipid vesicles on silica-based substrates such as glass is a common method used to fabricate supported lipid bilayers. Successful bilayer formation depends on a number of experimental conditions as well as on the quality of the vesicle preparation. Inevitably, a small fraction of unruptured vesicles always remains in a supported bilayer, and this kind of defect can have devastating influences on the morphological and electrical properties of the supported bilayer when used as a biosensing platform. In this paper, a simple method is reported to improve the completeness of supported bilayers by adding a vesicle rupturing peptide as a final step in the fabrication process. Peptide treatment reduces the fraction of unruptured vesicles to less than 1%, as determined by epifluorescence microscopy and quartz crystal microbalance-dissipation experiments. This step can easily be incorporated into existing procedures for preparing high-quality supported lipid bilayers. |
| URL الوصول: | https://research.chalmers.se/publication/233295 http://dx.doi.org/10.1039/c5cp06472d |
| قاعدة البيانات: | SwePub |
| تدمد: | 14639084 14639076 |
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| DOI: | 10.1039/c5cp06472d |