Removal of hypersignaling endosomes by simaphagy
| العنوان: | Removal of hypersignaling endosomes by simaphagy |
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| المؤلفون: | Migliano, Simona M., Schultz, Sebastian W., Wenzel, Eva M., Takáts, Szabolcs, Liu, Dan, Mørk, Silje, Tan, Kia Wee, Rusten, Tor Erik, Raiborg, Camilla, Stenmark, Harald |
| المصدر: | Autophagy. 20(4):769-791 |
| مصطلحات موضوعية: | Autophagy, endosome, ESCRT, receptor degradation, signaling |
| الوصف: | Activated transmembrane receptors continue to signal following endocytosis and are only silenced upon ESCRT-mediated internalization of the receptors into intralumenal vesicles (ILVs) of the endosomes. Accordingly, endosomes with dysfunctional receptor internalization into ILVs can cause sustained receptor signaling which has been implicated in cancer progression. Here, we describe a surveillance mechanism that allows cells to detect and clear physically intact endosomes with aberrant receptor accumulation and elevated signaling. Proximity biotinylation and proteomics analyses of ESCRT-0 defective endosomes revealed a strong enrichment of the ubiquitin-binding macroautophagy/autophagy receptors SQSTM1 and NBR1, a phenotype that was confirmed in cell culture and fly tissue. Live cell microscopy demonstrated that loss of the ESCRT-0 subunit HGS/HRS or the ESCRT-I subunit VPS37 led to high levels of ubiquitinated and phosphorylated receptors on endosomes. This was accompanied by dynamic recruitment of NBR1 and SQSTM1 as well as proteins involved in autophagy initiation and autophagosome biogenesis. Light microscopy and electron tomography revealed that endosomes with intact limiting membrane, but aberrant receptor downregulation were engulfed by phagophores. Inhibition of autophagy caused increased intra- and intercellular signaling and directed cell migration. We conclude that dysfunctional endosomes are surveyed and cleared by an autophagic process, simaphagy, which serves as a failsafe mechanism in signal termination. |
| وصف الملف: | electronic |
| URL الوصول: | https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-530028 https://doi.org/10.1080/15548627.2023.2267958 https://uu.diva-portal.org/smash/get/diva2:1865269/FULLTEXT01.pdf |
| قاعدة البيانات: | SwePub |
| DOI: | 10.1080/15548627.2023.2267958 |
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