An alternative cytokinin biosynthesis pathway
| العنوان: | An alternative cytokinin biosynthesis pathway |
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| المؤلفون: | Åstot, C, Dolezal, K, Nordström, A, Wang, Q, Kunkel, T, Moritz, T, Chua, N H, Sandberg, G |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America. 97(26):14778-14783 |
| الوصف: | Studies of de novo cytokinin biosynthesis in isopentenyltransferase (ipt)-transformed Arabidopsis thaliana, involving in vivo deuterium labeling and mass spectrometry, showed that the biosynthetic rate of zeatinriboside-5'-monophosphate was around 66-fold higher than that of isopentenyladenosine-5'-monophosphate (iPMP), the proposed primary product of the Agrobacterium ipt. Double tracer analysis, using [(2)H(6)] isopentenyladenosine and deuterium oxide, provided evidence for an alternative, iPMP-independent, biosynthetic pathway for zeatin-type cytokinins, present in both ipt-expressing and wild-type Arabidopsis thaliana. Reduction of the biosynthetic flux in the alternative pathway by use of mevastatin, an inhibitor for 3-hydroxy-3-methylglutaryl CoA reductase, indicated a terpenoid origin for the side-chain precursor of the iPMP independent pathway. |
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| URL الوصول: | https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-48254 https://doi.org/10.1073/pnas.260504097 |
| قاعدة البيانات: | SwePub |
| تدمد: | 00278424 10916490 |
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| DOI: | 10.1073/pnas.260504097 |