Bacteria Detection at a Single-Cell Level through a Cyanotype-Based Photochemical Reaction
| العنوان: | Bacteria Detection at a Single-Cell Level through a Cyanotype-Based Photochemical Reaction |
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| المؤلفون: | Dietvorst, Jiri, Ferrer-Vilanova, Amparo, Iyengar, Sharath Narayana, Russom, Aman, Prof., 1976, Vigues, Nuria, Mas, Jordi, Vilaplana, Lluisa, Marco, Maria-Pilar, Guirado, Gonzalo, Munoz-Berbel, Xavier |
| المصدر: | Analytical Chemistry. 94(2):787-792 |
| الوصف: | The detection of living organisms at very low concentrations is necessary for the early diagnosis of bacterial infections, but it is still challenging as there is a need for signal amplification. Cell culture, nucleic acid amplification, or nano-structure-based signal enhancement are the most common amplification methods, relying on long, tedious, complex, or expensive procedures. Here, we present a cyanotype-based photochemical amplification reaction enabling the detection of low bacterial concentrations up to a single-cell level. Photocatalysis is induced with visible light and requires bacterial metabolism of iron-based compounds to produce Prussian Blue. Bacterial activity is thus detected through the formation of an observable blue precipitate within 3 h of the reaction, which corresponds to the concentration of living organisms. The short time-to-result and simplicity of the reaction are expected to strongly impact the clinical diagnosis of infectious diseases. |
| وصف الملف: | |
| URL الوصول: | https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-311927 https://doi.org/10.1021/acs.analchem.1c03326 |
| قاعدة البيانات: | SwePub |
| تدمد: | 00032700 15206882 |
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| DOI: | 10.1021/acs.analchem.1c03326 |