Cyclic Expansion/Compression of the Air-Liquid Interface as a Simple Method to Produce Silk Fibers.
| العنوان: | Cyclic Expansion/Compression of the Air-Liquid Interface as a Simple Method to Produce Silk Fibers. |
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| المؤلفون: | Kvick, Mathias, Panagiotis Tasiopoulos, Christos, Barth, Andreas, Söderberg, Daniel, Lundell, Fredrik, Hedhammar, My, Professor, 1975 |
| المصدر: | Macromolecular Bioscience. 21(1) |
| مصطلحات موضوعية: | fibers, interfaces, proteins, silk |
| الوصف: | Self-assembly of recombinant spider silk protein at air-liquid interfaces is used as a starting point to produce homogeneous fiber bundles. The film that is formed on a silk protein solution in a vertically placed syringe is subjected to repeated controlled extension and compression by an oscillating vertical motion. Thereby, a precise breakup of the film can be achieved, followed by transport and roll-up against the syringe wall prior to extraction. Advantages of the method are that it 1) is simple to use; 2) requires a small volume of protein solution (1 mL) at relatively low concentration (1 mg mL-1 ); 3) can be performed under sterile conditions; 4) does not require any use of coagulants; and 5) is compatible with the addition of viable cells during the process, which thereby are integrated uniformly throughout the fiber. |
| وصف الملف: | |
| URL الوصول: | https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-283804 https://doi.org/10.1002/mabi.202000227 |
| قاعدة البيانات: | SwePub |
| تدمد: | 16165187 16165195 |
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| DOI: | 10.1002/mabi.202000227 |