Role of TRPM5 channel in insulin secretion from rat β-cells.: TRPM5 and insulin secretion
| العنوان: | Role of TRPM5 channel in insulin secretion from rat β-cells.: TRPM5 and insulin secretion |
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| المؤلفون: | Krishnan, Kalaiselvan, Ma, Zuheng, Björklund, Anneli, Islam, Md.Shahidul |
| المصدر: | Pancreas. 43(4):597-604 |
| مصطلحات موضوعية: | TRPM5, Islets of langerhans, calcium signaling, insulin secretion, Medical Technology, Medicinsk teknologi |
| الوصف: | OBJECTIVE:Several studies have reported that the transient receptor potential melastatin-like subtype 5 (TRPM5) channel, a Ca(2+)-activated monovalent cation channel, is involved in the stimulus-secretion coupling in the mouse pancreatic β-cells. We have studied the role of the TRPM5 channel in regulating insulin secretion and cytoplasmic free Ca(2+) concentration ([Ca(2+)](i)) in the rat β-cells by using triphenylphosphine oxide, a selective inhibitor of the channel.METHODS:Insulin secretion from islets from Sprague-Dawley rats was measured in batch incubations. Cytoplasmic free Ca(2+) concentration was measured from single β-cells by fura-2-based microfluorometry.RESULTS:Triphenylphosphine oxide did not alter insulin secretion and [Ca(2+)](i) response triggered by KCl or fructose. It inhibited insulin secretion in response to glucose, L-arginine, and glucagon-like peptide 1. It also inhibited glucose-induced insulin secretion by mechanisms that are independent of the adenosine triphosphate-sensitive potassium channels and [Ca(2+)](i) increase.CONCLUSIONS:Our results suggest that in the rat islets, TRPM5 is involved in mediating insulin secretion by glucose and l-arginine and in potentiating the glucose-induced insulin secretion by glucagon-like peptide 1. |
| وصف الملف: | electronic |
| URL الوصول: | https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-204559 https://kth.diva-portal.org/smash/get/diva2:1085183/FULLTEXT01.pdf |
| قاعدة البيانات: | SwePub |
| تدمد: | 08853177 15364828 |
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