التفاصيل البيبلوغرافية
| العنوان: |
Label-Free Fluctuation Spectroscopy Based on Coherent Anti-Stokes Raman Scattering from Bulk Water Molecules |
| المؤلفون: |
Rabasovic, M. D., Sisamakis, Evangelos, Wennmalm, Stefan, Widengren, Jerker |
| المصدر: |
ChemPhysChem. 17(7):1025-1033 |
| مصطلحات موضوعية: |
CARS (coherent anti-Stokes Raman scattering), FCS (fluorescence correlationspectroscopy), label-free, microparticles, nanoparticles |
| الوصف: |
Nanoparticles (NPs) and molecules can be analyzed by inverse fluorescence correlation spectroscopy (iFCS) as they pass through an open detection volume, displacing fractions of the fluorescence-emitting solution in which they are dissolved. iFCS does not require the NPs or molecules to be labeled. However, fluorophores in m-mm concentrations are needed for the solution signal. Here, we instead use coherent anti-Stokes Raman scattering (CARS) from plain water molecules as the signal from the solution. By this fully label-free approach, termed inverse CARS-based correlation spectroscopy (iCARS-CS), NPs that are a few tenths of nm in diameter and at pM concentrations can be analyzed, and their absolute volumes/concentrations can be determined. Likewise, lipid vesicles can be analyzed as they diffuse/flow through the detection volume by using CARS fluctuations from the surrounding water molecules. iCARS-CS could likely offer a broadly applicable, label-free characterization technique of, for example, NPs, small lipid exosomes, or microparticles in biomolecular diagnostics and screening, and can also utilize CARS signals from biologically relevant media other than water. |
| وصف الملف: |
print |
| URL الوصول: |
https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-185982 |
| قاعدة البيانات: |
SwePub |