التفاصيل البيبلوغرافية
| العنوان: |
C57BL/6 mice need MHC class II Aq to develop collagen-induced arthritis dependent on autoreactive T cells |
| المؤلفون: |
Backlund, J., Li, C., Jansson, E., Carlsen, S., Merky, P., Nandakumar, Kutty Selva, 1965, Haag, S., Ytterberg, J., Zubarev, R. A., Holmdahl, R. |
| المصدر: |
Annals of the Rheumatic Diseases. 72(7):1225-1232 |
| مصطلحات موضوعية: |
Animals, Arthritis, Experimental/*genetics/immunology, Rheumatoid/*genetics/immunology, Chickens, Collagen Type II/*immunology, *Disease Models, Animal, Epitopes, T-Lymphocyte/immunology, Genes, MHC Class II/*genetics, Haplotypes, Immunization, Mice, Congenic, Inbred C57BL, Inbred Strains, Mycobacterium/immunology, Rats, T-Lymphocytes/*immunology, Autoimmune Diseases, Inflammation, T Cells |
| الوصف: |
INTRODUCTION: Collagen-induced arthritis (CIA) has traditionally been performed in MHC class II A(q)-expressing mice, whereas most genetically modified mice are on the C57BL/6 background (expressing the b haplotype of the major histocompatibility complex (MHC) class II region). However, C57BL/6 mice develop arthritis after immunisation with chicken-derived collagen type II (CII), but arthritis susceptibility has been variable, and the immune specificity has not been clarified. OBJECTIVE: To establish a CIA model on the C57BL/6 background with a more predictable and defined immune response to CII. RESULTS: Both chicken and rat CII were arthritogenic in C57BL/6 mice provided they were introduced with high doses of Mycobacterium tuberculosis adjuvant. However, contaminating pepsin was strongly immunogenic and was essential for arthritis development. H-2(b)-restricted T cell epitopes on chicken or rat CII could not be identified, but expression of A(q) on the C57BL/6 background induced T cell response to the CII260-270 epitope, and also prolonged the arthritis to be more chronic. CONCLUSIONS: The putative (auto)antigen and its arthritogenic determinants in C57BL/6 mice remains undisclosed, questioning the value of the model for addressing T cell-driven pathological pathways in arthritis. To circumvent this impediment, we recommend MHC class II congenic C57BL/6N.Q mice, expressing A(q), with which T cell determinants have been thoroughly characterised. |
| وصف الملف: |
print |
| URL الوصول: |
https://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-48875 |
| قاعدة البيانات: |
SwePub |