Prevalence and molecular characterization of Cryptosporidium spp. in Père David’s deer (Elaphurus davidianus) in Jiangsu, China

التفاصيل البيبلوغرافية
العنوان: Prevalence and molecular characterization of Cryptosporidium spp. in Père David’s deer (Elaphurus davidianus) in Jiangsu, China
المؤلفون: Huang, Si-Yang, Fan, Yi-Min, Yang, Yi, Ren, Yi-Jun, Gong, Jing-Zhi, Yao, Na, Yang, Bin
المصدر: Revista Brasileira de Parasitologia Veterinária. January 2020 29(2)
بيانات النشر: Colégio Brasileiro de Parasitologia Veterinária, 2020.
سنة النشر: 2020
مصطلحات موضوعية: Cryptosporidium, Père David’s deer, 60 kDa glycoprotein (gp60) gene, the small subunit ribosomal RNA (SSU rRNA)
الوصف: Cryptosporidium is a zoonotic parasite that causes diarrhea in a broad range of animals, including deer. Little is known about the prevalence and genotype of Cryptosporidium spp. in Père David’s deer. In this study, 137 fecal samples from Père David’s deer were collected between July 2017 and August 2018 in the Dafeng Reserve and analyzed for Cryptosporidium spp. by nested-PCR based on the small subunit ribosomal RNA (SSU rRNA) gene, followed by sequence analyses to determine the species. The 60 kDa glycoprotein (gp60) gene was used to characterize Cryptosporidium spp. Among 137 samples, 2 (1.46%) were positive for Cryptosporidium spp. according to SSU rRNA gene sequencing results. Both samples belonged to the Cryptosporidium deer genotype, with two nucleotide deletions and one nucleotide substitution. The prevalence data and molecular characterization of this study provide basic knowledge for controlling and preventing Cryptosporidium infections in Père David’s deer in this area.
نوع الوثيقة: other
وصف الملف: text/html
اللغة: English
تدمد: 1984-2961
DOI: 10.1590/s1984-29612020013
URL الوصول: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612020000200401
Rights: info:eu-repo/semantics/openAccess
رقم الانضمام: edssci.S1984.29612020000200401
قاعدة البيانات: SciELO
الوصف
تدمد:19842961
DOI:10.1590/s1984-29612020013