التفاصيل البيبلوغرافية
| العنوان: |
Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil |
| المؤلفون: |
Aranha, Silvia de Araujo, Albuquerque, Leonardo Cunha de, Boiteux, Leonardo Silva, Inoue-Nagata, Alice Kazuko |
| المصدر: |
Tropical Plant Pathology. February 2011 36(1) |
| بيانات النشر: |
Sociedade Brasileira de Fitopatologia, 2011. |
| سنة النشر: |
2011 |
| مصطلحات موضوعية: |
Sida micrantha mosaic virus, geminivirus, SimMV |
| الوصف: |
A survey of okra begomoviruses was carried out in Central Brazil. Foliar samples were collected in okra production fields and tested by using begomovirus universal primers. Begomovirus infection was confirmed in only one (#5157) out of 196 samples. Total DNA was subjected to PCR amplification and introduced into okra seedlings by a biolistic method; the bombarded DNA sample was infectious to okra plants. The DNA-A and DNA-B of isolate #5157 were cloned and their nucleotide sequences exhibited typical characteristics of New World bipartite begomoviruses. The DNA-A sequence shared 95.6% nucleotide identity with an isolate of Sida micrantha mosaic virus from Brazil and thus identified as its okra strain. The clones derived from #5157 were infectious to okra, Sida santaremnensis and to a group of Solanaceae plants when inoculated by biolistics after circularization of the isolated insert, followed by rolling circle amplification. |
| نوع الوثيقة: |
article |
| وصف الملف: |
text/html |
| اللغة: |
English |
| تدمد: |
1982-5676 |
| DOI: |
10.1590/S1982-56762011000100002 |
| URL الوصول: |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000100002 |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edssci.S1982.56762011000100002 |
| قاعدة البيانات: |
SciELO |