التفاصيل البيبلوغرافية
| العنوان: |
Isolation and characterization of novel thermophilic lipase-secreting bacteria |
| المؤلفون: |
Rabbani, Mohammed, Bagherinejad, Mohammad Reza, Sadeghi, Hamid MirMohammad, Shariat, Ziaedin Samsam, Etemadifar, Zahra, Moazen, Fatemeh, Rahbari, Manizheh, Mafakher, Ladan, Zaghian, Saeideh |
| المصدر: |
Brazilian Journal of Microbiology. December 2013 44(4) |
| بيانات النشر: |
Sociedade Brasileira de Microbiologia, 2013. |
| سنة النشر: |
2013 |
| مصطلحات موضوعية: |
extracellular lipase, pH-stat method, thermophilic lipase, fermentation |
| الوصف: |
The purpose of the present study was to screen and identify the lipase-producing microorganisms from various regions of Iran. Samples collected from hot spring, Persian Gulf, desert area and oil-contaminated soil, were analyzed for thermophilic extracellular-lipase producing organisms. Six strains with high activity on rhodamine B plates were selected for chemical identification and further study. Among these isolated bacteria, four strains show higher activity in pH-Stat method at 55 °C. These strains were identified by PCR amplification of 16s rRNA genes using universal primers. Fermentation increased the activity up to 50%. The growth medium, designed for lipase production, increased the activity up to 4.55 folds. The crude supernatant of ZR-5 after fermentation and separation the cells, was lyophilized and the activity was measured. Total activity of this strain was 12 kU/g that shows its potential for industrial uses. Further study is required for purification of enzyme and calculation its specific activity. Immobilization is another approach should be considered. |
| نوع الوثيقة: |
article |
| وصف الملف: |
text/html |
| اللغة: |
English |
| تدمد: |
1517-8382 |
| DOI: |
10.1590/S1517-83822013000400013 |
| URL الوصول: |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400013 |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edssci.S1517.83822013000400013 |
| قاعدة البيانات: |
SciELO |
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