التفاصيل البيبلوغرافية
| العنوان: |
Isolation and partial purification of beta-galactosidases from cotyledons of two cowpea cultivars |
| المؤلفون: |
ENÉAS-FILHO, JOAQUIM, BARBOSA, GISLAINY KARLA DA COSTA, SUDÉRIO, FABRÍCIO BONFIM, PRISCO, JOSÉ TARQUÍNIO, GOMES-FILHO, ENÉAS |
| المصدر: |
Revista Brasileira de Fisiologia Vegetal. January 2001 13(3) |
| بيانات النشر: |
Sociedade Brasileira de Fisiologia Vegetal, 2001. |
| سنة النشر: |
2001 |
| مصطلحات موضوعية: |
cotyledons, isozymes, Vigna unguiculata, Vita 3 and Vita 5 cultivars, salt tolerance |
| الوصف: |
Three isoforms of beta-galactosidases were isolated and partially purified from the cotyledons of quiescent seeds of Vita 3 and Vita 5 cowpea [Vigna unguiculata (L.) Walp.] cultivars differing in water and salt stress tolerance. The purification procedure consisted of ammonium sulfate fractionation, acid precipitation, ion exchange chromatography through DEAE-sephadex and affinity chromatography through Lactosyl-sepharose columns. The three isoforms isolated from the two cultivars showed the same chromatographic patterns, same optimum of temperature for enzyme activity assay (60ºC), identical thermal stability up to 50°C, and similar pH optima (3-4). However, they differed from each other in sensitivity towards metal ions and certain chemical agents presents in the assay medium. The results have shown that the observed differences in beta-galactosidases from the cotyledons of quiescent seeds were not sufficient to relate them to stress tolerance. |
| نوع الوثيقة: |
article |
| وصف الملف: |
text/html |
| اللغة: |
English |
| تدمد: |
0103-3131 |
| DOI: |
10.1590/S0103-31312001000300001 |
| URL الوصول: |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000300001 |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edssci.S0103.31312001000300001 |
| قاعدة البيانات: |
SciELO |