التفاصيل البيبلوغرافية
| العنوان: |
DACT1 Involvement in the Cytoskeletal Arrangement of Cardiomyocytes in Atrial Fibrillation by Regulating Cx43 |
| المؤلفون: |
Hou, Jian, Yue, Yuan, Hu, Bo, Xu, Guangtao, Su, Ruibing, Lv, Linhua, Huang, Jiaxing, Yao, Jianping, Guan, Yuanjun, Wang, Keke, Wu, Zhongkai |
| المصدر: |
Brazilian Journal of Cardiovascular Surgery. December 2019 34(6) |
| بيانات النشر: |
Sociedade Brasileira de Cirurgia Cardiovascular, 2019. |
| سنة النشر: |
2019 |
| مصطلحات موضوعية: |
Atrial Fibrillation, Myocardium, Cytoskeleton, Actins, Myocytes, Cardiac, Connexin 43, Flow Cytometry, Western Blotting |
| الوصف: |
Objective: To determine the role of the dishevelled binding antagonist of beta catenin 1 (DACT1) in the cytoskeletal arrangement of cardiomyocytes in atrial fibrillation (AF). Methods: The DACT1 expression and its associations with the degree of fibrosis and β-catenin in valvular disease patients were analyzed by immunohistochemistry and Masson’s staining. DACT1 was overexpressed in the atrial myocyte cell line (HL-1) and the cardiac cell line (H9C2) by adenoviral vectors. Alterations in the fibrous actin (F-actin) content and organization and the expression of β-catenin were detected by flow cytometry, immunofluorescence, and Western blotting. Additionally, the association of DACT1 with gap junctions connexin 43 (Cx43) was detected by immunohistochemistry, immunofluorescence, and Western blotting. Results: Decreased cytoplasmic DACT1 expression in the myocardium was associated with AF (P=0.037) and a high degree of fibrosis (weak vs. strong, P=0.028; weak vs. very strong, P=0.029). A positive association was observed between DACT1 and β-catenin expression in clinical samples (P=0.028, Spearman’s rho=0.408). Furthermore, overexpression of DACT1 in HL-1 and H9C2 cells induced an increase in β-catenin and subsequent partial colocalization of DACT1 and β-catenin. In addition, F-actin content and organization were enhanced. Interestingly, DACT1 was positively correlated with the Cx43 expression in clinical samples (P=0.048, Spearman’s rho=0.370) and changed the Cx43 distribution in cardiac cell lines. Conclusion: DACT1 proved to be a novel AF-related gene by regulating Cx43 via cytoskeletal organization induced by β-catenin accumulation in cardiomyocytes. DACT1 could thus serve as a potential therapeutic marker for AF. |
| نوع الوثيقة: |
article |
| وصف الملف: |
text/html |
| اللغة: |
English |
| تدمد: |
0102-7638 |
| DOI: |
10.21470/1678-9741-2019-0033 |
| URL الوصول: |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382019000600012 |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edssci.S0102.76382019000600012 |
| قاعدة البيانات: |
SciELO |
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