التفاصيل البيبلوغرافية
| العنوان: |
Isolation and quantitative HPLC-PDA analysis of lupeol in phytopharmaceutical intermediate products from Vernonanthura ferruginea (Less.) H. Rob. |
| المؤلفون: |
Oliveira, Ezequiane Machado Silva, Freitas, Sabrine Lemes, Martins, Frederico Severino, Couto, Renê Oliveira, Pinto, Mariana Viana, Paula, José Realino, Conceição, Edemilson Cardoso, Bara, Maria Teresa Freitas |
| المصدر: |
Química Nova. January 2012 35(5) |
| بيانات النشر: |
Sociedade Brasileira de Química, 2012. |
| سنة النشر: |
2012 |
| مصطلحات موضوعية: |
quality control, triterpene, standardized dried extract |
| الوصف: |
Prior to obtain a standardized dried extract from V. ferruginea, lupeol was first time isolated from leaves and used as chemical maker. An analytical method using HPLC-PDA for lupeol determination in V. ferruginea intermediate products was developed using a C8 reverse-phase column, acetonitrile-acetic acid (99.99:0.01, v/v) as mobile phase at 0.8 mL min-1, oven temperature at 23-25 ºC, sample injection volume at 30 µL and detection at 210 nm. The method presented linearity from 10 to 160 µg mL-1, accuracy, precision, robustness and suitable sensitivity proving to be a useful tool to the obtainment process of lupeol standardized dried extracts of V. ferruginea. |
| نوع الوثيقة: |
report |
| وصف الملف: |
text/html |
| اللغة: |
English |
| تدمد: |
0100-4042 |
| DOI: |
10.1590/S0100-40422012000500032 |
| URL الوصول: |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000500032 |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edssci.S0100.40422012000500032 |
| قاعدة البيانات: |
SciELO |
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