التفاصيل البيبلوغرافية
| العنوان: |
Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea |
| Alternate Title: |
A cross-sectional study in four provinces of Mozambique: Diarrheagenic Escherichia coli in Mozambique |
| المؤلفون: |
Manhique-Coutinho, Lena, Chiani, Paola, Michelacci, Valeria, Taviani, Elisa, Bauhofer, Adilson Fernando Loforte, Chissaque, Assucênio, Cossa-Moiane, Idalécia, Sambo, Júlia, Chilaúle, Jorfélia, Guimarães, Esperança Lourenço, Salência, Judite, Cassocera, Marta, Bero, Diocreciano Matias, Langa, José Paulo, de Deus, Nilsa |
| المساهمون: |
Instituto de Higiene e Medicina Tropical (IHMT), RUN |
| سنة النشر: |
2022 |
| مصطلحات موضوعية: |
Antibiotics resistance, Children, Diarrheagenic E. coli pathotypes, Escherichia coli, Mozambique, R Medicine, QR Microbiology, RM Therapeutics. Pharmacology, RS Pharmacy and materia medica, Microbiology (medical), Infectious Diseases, SDG 3 - Good Health and Well-being |
| الوصف: |
Funding Information: The National Surveillance of Diarrhea was supported by a Senior Fellowship awarded to Nilsa de Deus by the European Foundations Initiative for African Research into Neglected Tropical Diseases (EFINTD, grant number 98539), the World Health Organization, a Master Fellowship funded by the Italian Agency for Development Cooperation (AICS) project AID10524, Deutsche Forschungsgemeinschaft (DFG, grant number JO369/5–1)—where Adilson Fernando Loforte Bauhofer and Assucênio Chissaque are fellows, GAVI (Global Alliance for Vaccines and Immunization) through Health System Strengthening (HSS), and Fundo Nacional de Investigação (FNI). The protocol was approved by the National Bioethics Committee for Health of Mozambique (IRB00002657, reference number: 348/CNBS/13), and each caregiver gave written informed consent to authorize their child's participation. The authors want to thank the parents or guardians who consented to their children's enrollment in the surveillance program. The authors acknowledge Dr. Octávio Jossai, the National Reference Laboratory of Microbiology team, all the focal points, and the provincial field teams who helped to conduct this study. Funding Information: The National Surveillance of Diarrhea was supported by a Senior Fellowship awarded to Nilsa de Deus by the European Foundations Initiative for African Research into Neglected Tropical Diseases (EFINTD, grant number 98539), the World Health Organization, a Master Fellowship funded by the Italian Agency for Development Cooperation (AICS) project AID10524, Deutsche Forschungsgemeinschaft (DFG, grant number JO369/5–1)—where Adilson Fernando Loforte Bauhofer and Assucênio Chissaque are fellows, GAVI (Global Alliance for Vaccines and Immunization) through Health System Strengthening (HSS), and Fundo Nacional de Investigação (FNI). Publisher Copyright: © 2022 |
| Description (Translated): |
Objectives: Analyze the frequency of diarrheagenic Escherichia coli (DEC) pathotypes and their antimicrobial resistance profiles among children aged <15 years with diarrhea in four Mozambican provinces. Methods: A cross-sectional hospital-based surveillance program of diarrhea was implemented in Maputo, Sofala, Zambézia, and Nampula. A single stool sample was collected from each child from May 2014 to May 2017. Culture methods and biochemical characterization were performed to detect E. coli strains. DEC pathotypes were determined by conventional polymerase chain reaction targeting specific virulence genes. Antimicrobial susceptibility was assessed by the Kirby–Bauer method. Results: From 723 specimens analyzed by culture, 262 were positive for E. coli. A total of 208 samples were tested by polymerase chain reaction for DEC identification, of which 101 (48.6%) were positive for a DEC pathotype. The predominant pathotypes were enteroaggregative (66.3%, 67/101), enteropathogenic (15.8%, 16/101), enterotoxigenic (13.9%, 14/101), and enteroinvasive E. coli (4.0%, 4/101). No Shiga toxin–producing E. coli was identified. Regardless of the province, the most frequent pathotype was enteroaggregative E. coli. Isolated DEC presented high frequency of resistance to ampicillin (97.8%), tetracycline (68.3%), chloramphenicol (28.4%), nalidixic acid (19.5%), and gentamicin (14.4%). Conclusion: Children with diarrhea in Mozambique had DEC and higher resistance to ampicillin and tetracycline. |
| نوع الوثيقة: |
journal article |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
1201-9712; PURE: 50808284 |
| DOI: |
10.1016/j.ijid.2022.04.054 |
| الاتاحة: |
http://hdl.handle.net/10362/148285 |
| Rights: |
open access |
| رقم الانضمام: |
rcaap.com.unl.run.unl.pt.10362.148285 |
| قاعدة البيانات: |
RCAAP |