Standardization of in vitro digestibility and DIAAS method based on the static INFOGEST protocol

التفاصيل البيبلوغرافية
العنوان: Standardization of in vitro digestibility and DIAAS method based on the static INFOGEST protocol
المؤلفون: Egger, Lotti, Sousa, Raquel, Recio, Isidra, Abbühl, Lychou, Brügger, Cédric, Hernández, Marta Santos, Macierzanka, Adam, Szumala, Patrycja, Rama, Addepalli, Mulet-Cabero, Ana Isabel, Perez-Moral, Natalia, Brodkorb, André, Fitzpatrick, Conor, Rieder, Anne, Levi, Carmit Shani, Gonçalves, Catarina, Amado, Isabel Rodriguez, Nobre, Clarisse, Pinheiro, A. C., Arranz, Elena, Bot, Francesca, Grundy, Myriam, Cruz, Igor Pacheco, Sepúlveda-González, Ailynne, Comi, Irene, Abrankó, László, Tormási, Judit, González, Felipe, Ruas-Madiedo, Patricia, Ruiz, Lorena, Faria, Miguel Ângelo, Sobral, M. Madalena C., Jiménez-Munoz, Luis Miguel, Corredig, Milena, Jimenez, Luis, Molly, Muleya, Rosa-Sibakov, Natalia, Ménard, Olivia, Orlien, Vibeke, Gousetti, Ourania, LykkePetersen, Iben, Ferranti, Pasquale, Olias, Raquel, Delgado-Andrade, Cristina, Assunção, Ricardo, Nicolas, Ruben Lopez, Simsek, Sebnem, Karakaya, Sibel, Nehir E, Sedef, Rezzi, Serge, Canarelli, Stéphane, Petit, Valérie, Tuncil, Yunus Emre, Hotrum, Nathalie, Broersen, Kerensa, Heimo, Dominique, Dubois, Sébastien, Portmann, Reto
المساهمون: Universidade do Minho
سنة النشر: 2022
الوصف: Background: The FAO recommends the digestible indispensable amino acid score (DIAAS) as the measure for protein quality, for which the true ileal digestibility needs to be assessed in humans or pigs. However, due to high costs and ethical concerns, the FAO strongly encourages as well the development of validated in vitro methods, which complement the in vivo experiments. Method: Recently, an in vitro workflow, based on the validated static INFOGEST protocol, was developed and compared towards in vivo data. In parallel to the validation with in vivo data, the repeatability and reproducibility of the in vitro protocol were tested in an international ring trial (RT) with the aim to establish an international ISO standard method within the International Dairy Federation (IDF). Five different dairy products (skim milk powder, whole milk powder, whey protein isolate, yoghurt, and cheese) were analyzed in 32 different laboratories from 18 different countries, across 4 continents. Results: in vitro protein digestibilities based on Nitrogen, free R-NH2, and total amino acids as well as DIAAS values were calculated and compared to in vivo data, where available. Conclusion: The in vitro method is suited for quantification of digestibility and will be further implemented to other food matrices
وصف الملف: application/pdf
اللغة: English
Relation: Egger, Lotti; Sousa, Raquel; Recio, Isidra; Abbühl, Lychou; Brügger, Cédric; Hernández, Marta Santos; Macierzanka, Adam; Szumala, Patrycja; Rama, Addepalli; Mulet-Cabero, Ana Isabel; Perez-Moral, Natalia; Brodkorb, André; Fitzpatrick, Conor; Rieder, Anne; Levi, Carmit Shani; Gonçalves, Catarina; Amado, Isabel Rodriguez; Nobre, Clarisse; Pinheiro, Ana Cristina; Arranz, Elena; Bot, Francesca; Grundy, Myriam; Cruz, Igor Pacheco; Sepúlveda-González, Ailynne; Comi, Irene; Abrankó, László; Tormási, Judit; González, Felipe; Ruas-Madiedo, Patricia; Ruiz, Lorena; Faria, Miguel Ângelo; Sobral, M. Madalena C.; Jiménez-Munoz, Luis Miguel; Corredig, Milena; Jimenez, Luis; Molly, Muleya; Rosa-Sibakov, Natalia; Ménard, Olivia; Orlien, Vibeke; Gousetti, Ourania; LykkePetersen, Iben; Ferranti, Pasquale; Olias, Raquel; Delgado-Andrade, Cristina; Assunção, Ricardo; Nicolas, Ruben Lopez; Simsek, Sebnem; Karakaya, Sibel; Nehir E, Sedef; Rezzi, Serge; Canarelli, Stéphane; Petit, Valérie; Tuncil, Yunus Emre; Hotrum, Nathalie; Broersen, Kerensa; Heimo, Dominique; Dubois, Sébastien; Portmann, Reto, Standardization of in vitro digestibility and DIAAS method based on the static INFOGEST protocol. ICFD 2022 - 7th International Conference on Food Digestion. Cork, Ireland, May 3-5, 21-22, 2022.; https://www.icfd2022.com/
الاتاحة: https://hdl.handle.net/1822/79234
Rights: open access
رقم الانضمام: rcaap.com.repositorium.repositorium.sdum.uminho.pt.1822.79234
قاعدة البيانات: RCAAP