Patent
Beta-glucan-rich lentinula edodes polysaccharide and preparation method thereof
| العنوان: | Beta-glucan-rich lentinula edodes polysaccharide and preparation method thereof |
|---|---|
| Patent Number: | 12202,913 |
| تاريخ النشر: | January 21, 2025 |
| Appl. No: | 18/813024 |
| Application Filed: | August 23, 2024 |
| مستخلص: | A preparation method of a Lentinula edodes polysaccharide rich in β-glucan includes: (1) extracting the Lentinula edodes polysaccharide to obtain a filtrate; (2) concentrating the filtrate to obtain a supernatant; (3) precipitating a crude Lentinula edodes polysaccharide from the supernatant using anhydrous ethanol to obtain a first precipitate; and (4) purifying the first precipitate to obtain the Lentinula edodes polysaccharide. The Lentinula edodes polysaccharide rich in the β-glucan has stable quality, and its yield, polysaccharide content and β-glucan content are significantly improved compared with water extraction and alcohol precipitation methods in the related art. In vitro pharmacological experiments prove that the Lentinula edodes polysaccharide rich in the β-glucan has good anti-inflammatory activity and can be applied to preparation of drugs or health foods. |
| Inventors: | SHANGHAI ACADEMY OF AGRICULTURAL SCIENCES (Shanghai, CN) |
| Assignees: | SHANGHAI ACADEMY OF AGRICULTURAL SCIENCES (Shanghai, CN) |
| Claim: | 1. A preparation method of a Lentinula edodes polysaccharide rich in β-glucan, comprising: (1) extracting the Lentinula edodes polysaccharide, comprising: using Lentinula edodes fruit bodies as raw material, pulverizing the raw material and then sieving the pulverized raw material through a 40-60 mesh sieve to obtain Lentinula edodes powder, adding the Lentinula edodes powder with water and an amylase to obtain a mixed solution, and heating the mixed solution to boiling and then keeping for 120-180 minutes (min) followed by filtering to obtain a filtrate; wherein a material-liquid ratio is 1 gram (g) of the Lentinula edodes powder: 15-20 milliliters (mL) of the water, and an addition amount of the amylase is 60-80 microliters (μL) of amylase solution per 1 g of the Lentinula edodes powder; (2) concentrating the filtrate, comprising: heating the filtrate to boiling and concentrating to obtain a concentrated solution with a material-liquid ratio of 1 g of the Lentinula edodes powder: 9-11 mL of the concentrated solution, centrifuging the concentrated solution at 8000 revolutions per minute (rpm) for 20 min to obtain a supernatant; (3) precipitating a crude Lentinula edodes polysaccharide using anhydrous ethanol, comprising: adding the anhydrous ethanol to the supernatant while stirring continuously until an ethanol content reaches 18-23% to obtain a mixture, standing the mixture at 4° C. for 10-12 hours and then centrifuging the mixture at 8000 rpm to remove an ethanol supernatant to obtain a first precipitate; and (4) purifying the first precipitate, comprising: adding 20-23% ethanol with a volume 5-8 times a volume of the first precipitate to the first precipitate and then shaking vigorously followed by centrifuging at 8000 rpm for 15 min to obtain a second precipitate, repeating above steps for the second precipitate to obtain a third precipitate, adding water to the third precipitate for dissolving to obtain a solution, evaporating the ethanol from the solution in a water bath at 80-100° C. followed by freeze-drying to obtain a gray-white cotton-like solid sample as the Lentinula edodes polysaccharide rich in the β-glucan. |
| Claim: | 2. The Lentinula edodes polysaccharide rich in the β-glucan prepared by the preparation method as claimed in claim 1 . |
| Patent References Cited: | |
| Other References: | Rao et al., Biocatalysis and Agricultural Biotechnology, 2021, 37, article 102163, 13 pages. (Year: 2021). cited by examiner Xue et al., Food Bioscience, 2019, 32, article 100452, 7 pages. (Year: 2019). cited by examiner |
| Primary Examiner: | Lau, Jonathan S |
| رقم الانضمام: | edspgr.12202913 |
| قاعدة البيانات: | USPTO Patent Grants |
| الوصف غير متاح. |