Patent
Methods of modulating seed and organ size in plants
| العنوان: | Methods of modulating seed and organ size in plants |
|---|---|
| Patent Number: | 11519,001 |
| تاريخ النشر: | December 06, 2022 |
| Appl. No: | 16/387196 |
| Application Filed: | April 17, 2019 |
| مستخلص: | This invention relates to a plant E3 ubiquitin ligase (termed DA2) which acts synergistically with DA1 to control seed and organ size. Methods of increasing plant yield are provided that comprise reducing the expression or activity of DA2 in a plant that is deficient in DA1 expression or activity. Plants with increased yield and methods of producing such plants are also provided. |
| Inventors: | INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY CHINESE ACADEMY OF SCIENCES (Beijing, CN); Plant Bioscience Limited (Norwich, GB) |
| Assignees: | INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY CHINESE ACADEMY OF SCIENCES (Beijing, CN), PLANT BIOSCIENCE LIMITED (Norwich, GB) |
| Claim: | 1. A method of increasing the biomass or seed yield of a plant comprising: introducing within cells of said plant a mutation in a nucleotide sequence encoding a DA2 polypeptide, wherein a wild-type DA2 polypeptide comprises an amino acid sequence having at least 95% sequence identity to one or more of SEQ ID NOs: 20 to 35, wherein the mutation abolishes the expression or activity of the DA2 polypeptide, and wherein the biomass or seed yield per plant is increased relative to a plant without the mutation in the nucleotide sequence encoding the DA2 polypeptide. |
| Claim: | 2. The method according to claim 1 wherein the plant also has (i) a mutation in a nucleotide sequence encoding a DA1 polypeptide, wherein a wild-type DA1 protein comprises an amino acid sequence having at least 95% sequence identity to one or more of SEQ ID NOs: 41 to 64, wherein the mutation abolishes DA1 expression or activity; and/or (ii) a mutation in a nucleotide sequence encoding an EOD1 polypeptide, wherein a wild-type EOD1 polypeptide comprises a sequence having at least 95% sequence identity to one or more of SEQ ID NOs: 74 to 90, wherein the mutation abolishes EOD1 expression or activity. |
| Claim: | 3. A method of increasing the biomass or seed yield of a plant comprising: introducing within cells of said plant a mutation in a nucleotide sequence encoding a DA2 polypeptide, wherein a wild-type DA2 polypeptide comprises an amino acid sequence having at least 95% sequence identity to one or more of SEQ ID NOs: 20 to 35, wherein the mutation abolishes the expression or activity of the DA2 polypeptide; introducing within cells of said plant (i) a mutation in a nucleotide sequence encoding a DA1 polypeptide, wherein a wild-type DA1 protein comprises an amino acid sequence having at least 95% sequence identity to one or more of SEQ ID NOs: 41 to 64, wherein the mutation abolishes the expression or activity of the DA1 polypeptide; and/or (ii) a mutation in a nucleotide sequence encoding an EOD1 polypeptide, wherein a wild-type EOD1 polypeptide comprises a sequence having at least 95% sequence identity to one or more of SEQ ID NOs: 74 to 90, wherein the mutation abolishes the expression or activity of the EOD1 polypeptide, and wherein the biomass or seed yield per plant is increased relative to a plant without the mutation in the nucleotide sequence encoding the DA2 polypeptide. |
| Claim: | 4. The method according to claim 1 wherein the plant has increased plant size, seed size and/or organ size relative to a plant without the mutation that reduces the expression or activity of the DA2 polypeptide. |
| Claim: | 5. The method according to claim 1 comprising sexually or asexually propagating or growing off-spring or descendants of the plant having reduced DA2 expression or activity. |
| Claim: | 6. The method according to claim 1 wherein the DA2 polypeptide comprises a RING domain of SEQ ID NO:2. |
| Claim: | 7. The method according to claim 6 wherein the DA2 polypeptide comprises SEQ ID NO: 36. |
| Claim: | 8. The method according to claim 6 wherein the DA2 polypeptide comprises SEQ ID NO: 37. |
| Claim: | 9. The method according to claim 2 wherein the DA1 polypeptide comprises a UIM1 domain of SEQ ID NO: 68 and a UIM2 domain of SEQ ID NO: 69. |
| Claim: | 10. The method according to claim 9 wherein the DA1 polypeptide comprises a LIM domain of SEQ ID NO: 38 or 39. |
| Claim: | 11. The method according to claim 1 wherein the plant is a higher plant. |
| Claim: | 12. The method according to claim 11 wherein the plant is an agricultural plant selected from the group consisting of Lithospermum erythrorhizon, Taxus spp, tobacco, cucurbits, carrot, vegetable brassica, melons, capsicums, grape vines, lettuce, strawberry, oilseed brassica, sugar beet, wheat, barley, maize, rice, soyabeans, peas, sorghum, sunflower, tomato, potato, pepper, chrysanthemum, carnation, linseed, hemp and rye. |
| Claim: | 13. A plant comprising: a mutation in a nucleotide sequence encoding a DA2 polypeptide, wherein a wild-type DA2 polypeptide comprises an amino acid sequence having at least 95% sequence identity to one or more of SEQ ID NOs: 20 to 35, wherein the mutation abolishes the expression or activity of the DA2 polypeptide; and a mutation in a nucleotide sequence encoding a DA1 polypeptide, wherein a wild-type DA1 protein comprises an amino acid sequence having at least 95% sequence identity to one or more of SEQ ID NOs: 41 to 64, wherein the mutation abolishes the expression or activity of the DA1 polypeptides and/or a mutation in a nucleotide sequence encoding a EOD1 polypeptide, wherein a wild-type EOD1 polypeptide comprises a sequence having at least 95% sequence identity to one or more of SEQ ID NOs: 74 to 90, wherein the mutation abolishes the expression or activity of the EOD1 polypeptide, wherein the biomass or seed yield per plant is increased relative to a plant without the mutation in the nucleotide sequence encoding the DA2 polypeptide. |
| Claim: | 14. A plant which is produced by the method according to claim 3 . |
| Patent References Cited: | 10329577 June 2019 Li 20110247094 October 2011 Allen |
| Other References: | Raab et al., The Plant Journal 59:39-51, 2009 (Year: 2009). cited by examiner Disch et al., “The E3 Ubiquitin Ligase Big Brother Controls Arabidopsis Organ Size in a Dosage-Dependent Manner”, Current Biology, vol. 16, pp. 272-279, Feb. 7, 2006. cited by applicant Du et al., “The Ubiquitin Receptor DA1 Regulates Seed and Organ Size by Modulating the Stability of the Ubiquitin-Specific Protease UBP15/SOD2 in Arabidopsis”, The Plant Cell, vol. 26, pp. 665-677, Feb. 2014. cited by applicant Guerra et al., “Identification of a Protein Network Interacting with TdRF1, a Wheat RING Ubiquitin Ligase with a Protective Role against Cellular Dehydration”, vol. 158, pp. 777-789, Feb. 2012. cited by applicant Krizek, Beth A, “Making bigger plants: key regulators of final organ size”, Current Opinion in Plant Biology, vol. 12, pp. 17-22, Jan. 1, 2009. cited by applicant Li et al., Control of final seed and organ size by the DA1 gene family in Arabidopsis thaliana, Genes Dev., vol. 22, pp. 1331-1336, Nov. 4, 2014. cited by applicant Van Daele et al., “A comparative study of seed yield parameters in Arabidopsis thaliana mutants and transgenics”, Plant Biotechnology Journal, vol. 10, pp. 488-500, Jan. 11, 2012. cited by applicant Xia et al., “The Ubiquitin Receptor DA1 Interacts with the E3 Ubiquitin Ligase DA2 to Regulate Seed and Organ Size in Arabidopsis”, The Plant Cell, vol. 25, pp. 3347-3359 Sep. 2013. cited by applicant Xia et al., “DA2 acts synergistically with DA1 to regulate plant seed and organ size”, Plant Molecular Biology and Modern Agriculture—Abstracts of Papers of National Plant Biology Seminar, Publication date Jul. 18, 2010. cited by applicant European Patent Office, “International Searching Report and Written Opinion”, issued in connection to International Application No. PCT/EP2014/066427, dated Oct. 30, 2014, 15 pages Oct. 30, 2014. cited by applicant |
| Primary Examiner: | McElwain, Elizabeth F |
| Attorney, Agent or Firm: | McKee, Voorhees & Sease, PLC |
| رقم الانضمام: | edspgr.11519001 |
| قاعدة البيانات: | USPTO Patent Grants |
| الوصف غير متاح. |