Patent
Enzyme-mediated assimilation of DNA-functionalized single-walled carbon nanotubes (SWNTs)
| العنوان: | Enzyme-mediated assimilation of DNA-functionalized single-walled carbon nanotubes (SWNTs) |
|---|---|
| Patent Number: | 10526,628 |
| تاريخ النشر: | January 07, 2020 |
| Appl. No: | 13/267002 |
| Application Filed: | October 06, 2011 |
| مستخلص: | Select embodiments of the present invention employ biological means to direct assemble CNT-based nanostructures, allowing for scaling to macrostructures for manufacture. In select embodiments of the present invention, a method is provided for assembling DNA-functionalized SWNTs by phosphodiester bonding catalyzed by ssDNA-ligase to form macroscopic CNT aggregates. |
| Inventors: | Arnett, Clint M. (Mahomet, IL, US); Marsh, Charles P. (Urbana, IL, US); Han, Jae Hee (Gyeonggi-do, KR); Strano, Michael S. (Lexington, MA, US); Welch, Charles R. (Vicksburg, MS, US); Carlson, Thomas A. (Champaign, IL, US) |
| Assignees: | UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE ARMY (Alexandria, VA, US) |
| Claim: | 1. A method for aggregating CNTs to permit scaling for manufacture of macro-scale items, comprising: functionalizing SWNTs; performing ligation reactions using an adenosine triphosphate (ATP)-dependent, thermostable ssDNA-ligase, wherein said ligation reactions are initiated in a heated water bath; and terminating said reactions after a pre-specified period by cooling said water bath to room temperature; and wherein said SWNTs are functionalized with single-stranded 30-mer polythymine oligonucleotides (d(T) 30) by ultra-sonication. |
| Claim: | 2. The method of claim 1 centrifuging said SWNTs to remove insoluble CNTs. |
| Claim: | 3. The method of claim 1 removing potentially unbound said ssDNA. |
| Claim: | 4. The method of claim 1 performing ligation reactions using: purified DNA-functionalized SWNTs, 10× ligase buffer, 1 mM ATP, said ssDNA-ligase, and molecular-grade water. |
| Claim: | 5. The method of claim 4 initiating said reactions by incubating in a water bath at a temperature of approximately 30° C. to approximately 100° C. |
| Claim: | 6. The method of claim 4 initiating said reactions by incubating in a water bath at a temperature of approximately 60° C. |
| Claim: | 7. The method of claim 5 terminating said reactions after approximately one hour by cooling to room temperature. |
| Patent References Cited: | 7566749 July 2009 Fischer et al. 7897209 March 2011 Shibuya et al. 7910650 March 2011 Fischer et al. 2007/0280876 December 2007 Tour et al. 2008/0134942 June 2008 Brenner et al. 2008/0213162 September 2008 Smalley et al. 2008/0233402 September 2008 Carlson et al. 2009/0232965 September 2009 Hata et al. 2010/0173153 July 2010 Hatta et al. 2010/0203316 August 2010 Hata et al. 2010/0308279 December 2010 Zhou et al. 2011/0008617 January 2011 Hata et al. 2011/0116995 May 2011 Shibuya et al. 2011/0133133 June 2011 Fischer et al. |
| Other References: | Martin, Willis, Wusheng Zhu, and Goran Krilov. “Simulation Study of Noncovalent Hybridization of Carbon Nanotubes by Single-Stranded DNA in Water” The Journal of Physical Chemistry B 112.50 (2008): 16076-16089. cited by examiner O'Neill, Patrick, et al. “Sturdier DNA nanotubes via ligation.” Nano letters 6.7 (2006): 1379-1383. cited by examiner Chen, Yi, et al. “DNA-directed assembly of single-wall carbon nanotubes.” Journal of the American Chemical Society 129.28 (2007): 8696-8697. cited by examiner Yarotski, Dzmitry A., et al. “Scanning tunneling microscopy of DNA-wrapped carbon nanotubes.” Nano letters 9.1 (2008): 12-17. cited by examiner Arnett et al., Enzyme-Mediated Assimilation of DNA-Functionalized Single-Walled Carbon Nanotubes (SWNT), American Chemical Society, Revised (unpublished) manuscript, Nov. 23, 2009. cited by applicant Babic et al., Suitability of CNTs grown by CVD for Electric Devices, Instit fur Physik, Universitat Basel, Basel, Switzerland, undated (pre-2009). cited by applicant |
| Primary Examiner: | Rump, Richard M |
| Attorney, Agent or Firm: | Jones, Brian C. |
| رقم الانضمام: | edspgr.10526628 |
| قاعدة البيانات: | USPTO Patent Grants |
| الوصف غير متاح. |