Patent
Detecting and treating breast cancer resistance to EGFR inhibitors
| العنوان: | Detecting and treating breast cancer resistance to EGFR inhibitors |
|---|---|
| Patent Number: | 9,303,088 |
| تاريخ النشر: | April 05, 2016 |
| Appl. No: | 13/248690 |
| Application Filed: | September 29, 2011 |
| مستخلص: | The application describes therapeutic compositions and methods for treating cancer. For example, therapeutic compositions and methods related to inhibition of FAM83A (family with sequence similarity 83) are provided. The application also describes methods for diagnosing cancer resistance to EGFR inhibitors. For example, a method of diagnosing cancer resistance to EGFR inhibitors by detecting increased FAM83A levels is described. |
| Inventors: | Moonlee, Sun-Young (Busan, KR); Bissell, Mina J. (Berkeley, CA, US); Furuta, Saori (Emeryville, CA, US); Meier, Roland (Geneva, CH); Kenny, Paraic A. (Yonkers, NY, US) |
| Assignees: | The Regents of the University of California (Oakland, CA, US) |
| Claim: | 1. A method of predicting a clinical outcome for a patient having breast cancer, comprising: providing a biological sample comprising breast cancer cells from the patient; contacting said breast cancer cells with an antibody specific for FAM83A; detecting a FAM83A expression level in the breast cancer cells by measuring the level of binding between the antibody and the breast cancer cells; and predicting the clinical outcome of the patient based on the FAM83A expression level, wherein the FAM83A expression level is positively correlated with the breast-cancer related mortality. |
| Claim: | 2. The method of claim 1 , wherein predicting the clinical outcome comprises generating a Kaplan-Merier survival curve. |
| Claim: | 3. The method of claim 1 , wherein the antibody specific for FAM83A is a polyclonal antibody against FAM83A. |
| Claim: | 4. The method of claim 3 , wherein the polyclonal antibody is raised against a peptide having an amino acid sequence of SEQ ID NO: 6. |
| Claim: | 5. The method of claim 3 , wherein the polyclonal antibody is raised against a peptide having an amino acid sequence of SEQ ID NO: 2. |
| Claim: | 6. The method of claim 1 , wherein the antibody specific for FAM83A protein is a monoclonal antibody against FAM83A. |
| Claim: | 7. The method of claim 1 , wherein high expression level of FAM83A in said patient is indicative of high clinical mortality as compared to patients carrying tumors with lower expression levels of FAM83A. |
| Claim: | 8. The method of claim 1 , wherein the biological sample is a biopsy sample from the patient. |
| Patent References Cited: | 2005/0106644 May 2005 Cairns et al. 2008/0113874 May 2008 Bunn et al. WO 97/00271 January 1997 WO 2008/104543 September 2008 |
| Other References: | Armstrong et al., “A Phase I Study of Chemically Synthesized Verotoxin (Shiga-like Toxin) Pk-Trisaccharide Receptors Attached to Chromosorb for Preventing Hemolytio-Uremic Syndrome” J. Infectious Diseases 171:1042-1045 (1995). cited by applicant International Search Report and Written Opinion dated Aug. 11, 2010 for PCT/US2010/029497 filed Mar. 31, 2010. cited by applicant Corkery et al. “Epidermal growth factor receptor as a potential therapeutic target in triple-negative breast cancer,” Annals of Oncology 20: 862-867 (2009), published online Jan. 15, 2009. cited by applicant Davidson et al., “Epidermal growth factor receptor gene expression in estrogen receptor-positive and negative human breast cancer cell lines,” Molecular Endocrinology, 1(3):216-223 (1987). cited by applicant Lee, Chapters 4 and 5 of “Identification and characterization of novel genes involved in signaling pathways that disrupt phenotypic reversion in a model of human breast cancer cells in 3-D IrECM cultures”, University of California, Berkeley, Dissertation for the degree of Doctor of Philosophy, Fall 2007. cited by applicant Lee et al. “FAM83A confers EGFR-TKI resistance in breast cancer cells and in mice,” Journal of Clinical Investigation, 122(9):3211-3220 (2012). cited by applicant |
| Primary Examiner: | Joike, Michele K |
| Attorney, Agent or Firm: | Knobbe Martens Olson & Bear LLP |
| رقم الانضمام: | edspgr.09303088 |
| قاعدة البيانات: | USPTO Patent Grants |
| الوصف غير متاح. |