Patent
Method for recombinant production of a desired polypeptide using a mammalian cell co-expressing a taurine transporter polypeptide
| العنوان: | Method for recombinant production of a desired polypeptide using a mammalian cell co-expressing a taurine transporter polypeptide |
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| Patent Number: | 9,238,687 |
| تاريخ النشر: | January 19, 2016 |
| Appl. No: | 12/226195 |
| Application Filed: | April 12, 2007 |
| مستخلص: | The present invention provides a method capable of producing a natural or recombinant protein at low cost. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses a taurine transporter and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide. Hamster taurine transporter, a DNA encoding the same, a recombinant vector and a transformed cell are also provided. |
| Inventors: | Tabuchi, Hisahiro (Tokyo, JP); Sugiyama, Tomoya (Tokyo, JP); Tanaka, Saeko (Tokyo, JP); Tainaka, Satoshi (Tokyo, JP) |
| Assignees: | Chugai Seiyaku Kabushiki Kaisha (Tokyo, JP) |
| Claim: | 1. A method of producing a desired secreted polypeptide comprising culturing an isolated mammalian cell transfected with a DNA encoding a taurine transporter and further transfected with a DNA encoding a desired secreted polypeptide, thereby allowing the mammalian cell to produce said desired secreted polypeptide, wherein the DNA encoding the taurine transporter is any one of the following (a) or (b): (a) a DNA encoding a polypeptide having the amino acid sequence of SEQ ID NO: 2; or (b) a DNA encoding a polypeptide having 98% or more amino acid sequence homology with the amino acid sequence of SEQ ID NO: 2, and wherein the taurine transporter has taurine transporter activity. |
| Claim: | 2. The method of claim 1 , wherein the isolated mammalian cell is a Chinese hamster ovary cell. |
| Claim: | 3. The method of claim 1 , comprising culturing the isolated mammalian cell in a medium with a taurine concentration of 0 to 100 g/L. |
| Claim: | 4. A method of preparing a pharmaceutical comprising a desired secreted polypeptide, comprising preparing the desired secreted polypeptide by the method of any one of claim 1 , 2 or 3 and preparing the pharmaceutical comprising the desired secreted polypeptide. |
| Claim: | 5. An isolated DNA comprising a DNA encoding a taurine transporter and a heterologous promoter, wherein the DNA encoding the taurine transporter is any one of the following (a) or (b): (a) a DNA encoding a polypeptide having the amino acid sequence of SEQ ID NO: 2; or (b) a DNA encoding a polypeptide having 98% or more amino acid sequence homology with the amino acid sequence of SEQ ID NO: 2, and wherein the taurine transporter has taurine transporter activity. |
| Claim: | 6. A recombinant vector comprising the isolated DNA of claim 5 . |
| Claim: | 7. An isolated cell transformed or transfected with the isolated DNA of claim 5 . |
| Claim: | 8. The method of claim 1 , wherein the desired secreted polypeptide is an antibody. |
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| Primary Examiner: | Steadman, David J |
| Attorney, Agent or Firm: | Foley & Lardner LLP |
| رقم الانضمام: | edspgr.09238687 |
| قاعدة البيانات: | USPTO Patent Grants |
| الوصف غير متاح. |