Patent
Cell counting and sample chamber and methods of fabrication
| العنوان: | Cell counting and sample chamber and methods of fabrication |
|---|---|
| Patent Number: | 8,928,876 |
| تاريخ النشر: | January 06, 2015 |
| Appl. No: | 13/519283 |
| Application Filed: | January 19, 2011 |
| مستخلص: | The invention generally relates to analytical and monitoring systems useful for analyzing and measuring cells and biological sample. More particularly, the invention relates to a unique cell counting chamber, e.g., a thin gap fluidic cell chamber for both bright field and fluorescent imaging of bacteria or parasites, and methods for making the same. |
| Inventors: | Chan, Leo L. (North Andover, MA, US); Qiu, Jean (North Andover, MA, US) |
| Assignees: | Nexcelom Bioscience LLC (Lawrence, MA, US) |
| Claim: | 1. A sample chamber suitable for holding a liquid sample for optical imaging, comprising: a top layer; an adhesive layer; a bottom layer; a sample holding cavity between and defined by the adhesive layer and the bottom layer having a fixed depth of from about 1 μm to about 100 μm; an inlet port having an opening in the top layer for placing a liquid sample into the chamber; and an outlet port having an opening in the top layer for removing the liquid sample out of the chamber; wherein at least a portion of the adhesive layer is optically clear in the range of about 250 nm to about 10,000 nm in wavelength, and wherein at least a portion of the top layer and the bottom layer is optically clear in the range of about 300 nm and about 10,000 nm in wavelength. |
| Claim: | 2. The sample chamber of claim 1 , wherein the bottom layer comprises a base film. |
| Claim: | 3. The sample chamber of claim 1 , wherein the top layer comprises a material selected from the group consisting of polyester, polycarbonate, PMMA, polystyrene and cyclic olefin copolymer. |
| Claim: | 4. The sample chamber of claim 1 , wherein the adhesive layer is a pressure-sensitive adhesive layer comprising a material selected from acrylic or acrylate adhesive. |
| Claim: | 5. The sample chamber of claim 1 , wherein the bottom layer comprises an irradiation or thermally cured material. |
| Claim: | 6. The sample chamber of claim 5 , wherein the bottom layer is an irradiation cured material. |
| Claim: | 7. The sample chamber of claim 6 , wherein the bottom layer is a UV-cured material. |
| Claim: | 8. The sample chamber of claim 7 , wherein the UV-cured materials is selected from the group consisting of UV-cured acrylate, urethane and epoxy. |
| Claim: | 9. The sample chamber of claim 1 , further comprising a backing layer bond to the bottom layer. |
| Claim: | 10. A sample chamber suitable for holding a biological sample for optical imaging, comprising: an optically clear bottom layer; an optically clear top layer; an optically clear adhesive layer binding the top layer on one side and forming a sample holding chamber on the other side with an irradiation or thermally cured polymer layer, wherein the sample holding chamber having a fixed depth, as defined by the depth from the adhesive layer to the irradiation- or thermally-cured polymer layer, of from about 1 μm to about 100 μm; a first opening in the optically clear top layer for introducing the sample into the chamber; and a second opening in the optically clear top layer for removing the sample from the chamber. |
| Claim: | 11. The sample chamber of claim 10 , wherein the optically adhesive layer is wherein at least a portion of the adhesive layer is optically clear in the range of about 250 nm to about 10,000 nm in wavelength. |
| Claim: | 12. The sample chamber of claim 10 , wherein the top layer comprises a material selected from the group consisting of polyester, polycarbonate, PMMA, polystyrene and cyclic olefin copolymer. |
| Claim: | 13. The sample chamber of claim 10 , wherein the adhesive layer is a pressure-sensitive adhesive layer comprising a material selected from acrylic or acrylate adhesive. |
| Claim: | 14. The sample chamber of claim 10 , wherein the bottom layer comprises an irradiation or thermally cured material. |
| Current U.S. Class: | 356/246 |
| Patent References Cited: | 4690503 September 1987 Janssen et al. 4761381 August 1988 Blatt et al. 6558630 May 2003 Degn et al. 2004/0145805 July 2004 Qiu 2005/0136260 June 2005 Garcia 2009/0062152 March 2009 Linton et al. |
| Assistant Examiner: | Rahman, Md |
| Primary Examiner: | Nur, Abdullahi |
| Attorney, Agent or Firm: | Milstein Zhang & Wu LLC |
| رقم الانضمام: | edspgr.08928876 |
| قاعدة البيانات: | USPTO Patent Grants |
| الوصف غير متاح. |