Patent
Synthetic gene control region
| العنوان: | Synthetic gene control region |
|---|---|
| Patent Number: | 8,026,082 |
| تاريخ النشر: | September 27, 2011 |
| Appl. No: | 11/921903 |
| Application Filed: | June 09, 2006 |
| مستخلص: | The present invention provides a synthetic gene control region which comprises a gene regulatory sequence comprising a binding site for a gene regulatory protein of a yeast strain, and a promoter from filamentous fungal strain located downstream of the gene regulatory sequence; wherein the promoter can be recognized by the general transcription factors and RNA polymerase of the yeast strain; wherein the gene regulatory sequence is capable of regulating transcription initiated by the filamentous fungal promoter in the yeast strain. |
| Inventors: | Bryan, Janine (Furlong, PA, US); Brownlow, Michelle K. (Jamison, PA, US); Schultz, Loren D. (Harleysville, PA, US); Losada, Maria C. (Union, NJ, US); Jansen, Kathrin Ute (Allendale, NJ, US); Kurtz, Myra (Martinsville, NJ, US); Kurtz, legal representative, Stuart (Martinsville, NJ, US) |
| Assignees: | Merck Sharp & Dohme Corp. (Rahway, NJ, US) |
| Claim: | 1. A synthetic gene control region comprising, a synthetic gene regulatory sequence comprising a binding site for a gene regulatory protein of a yeast strain, and a promoter from a filamentous fungal strain located downstream of the synthetic gene regulatory sequence; wherein the binding site is a binding site for GAL4 protein of S. cerevisiae , and the promoter is a promoter for the XynA gene of Penicillium purpurogenum , wherein the promoter can be recognized by the general transcription factors and RNA polymerase of the yeast strain; wherein the synthetic gene regulatory sequence is capable of regulating the transcription initiated by a filamentous fungal promoter in the yeast strain. |
| Claim: | 2. The control region of claim 1 wherein the binding site is not a sequence in the naturally-occurring gene regulatory regions of the yeast strain. |
| Claim: | 3. The control region of claim 1 wherein the binding site comprises a DNA sequence selected from the group consisting of SEQ ID NO: 8, 9, 10, 11, 12, and 15. |
| Claim: | 4. The control region of claim 3 wherein the gene regulatory sequence further comprises a binding site for a gene repressor. |
| Claim: | 5. The control region of claim 4 wherein the binding site for the gene repressor is a binding site for the MIG1 protein of S. cerevisiae. |
| Claim: | 6. The control region of claim 5 wherein the binding site for MIG1 protein comprises a sequence selected from the group consisting of SEQ ID NO: 18, 19, and 20. |
| Claim: | 7. The control region of claim 1 wherein the synthetic gene control region comprises a sequence selected from the group consisting of SEQ ID NO: 21, 22, 23, 24, 25, and 26. |
| Claim: | 8. A DNA expression vector comprising, a synthetic gene control region, wherein the synthetic gene control region comprises a sequence selected from the group consisting of SEQ ID NO:21, 22, 23, 24, 25, and 26; a coding sequence encoding a protein, polypeptide, or peptide under the control of the control region, and an yeast selection marker. |
| Claim: | 9. The DNA expression vector of claim 8 further comprising a polyadenylation signal sequence located downstream of the coding sequence. |
| Claim: | 10. The DNA expression vector of claim 8 further comprising a transcription terminator located downstream of the coding sequence. |
| Claim: | 11. The DNA expression vector of claim 8 wherein the yeast selection marker is a S. cerevisiae selection marker. |
| Claim: | 12. The DNA expression vector of claim 11 wherein the S. cerevisiae selection marker is selected from the group consisting of LEU2, TRP1, URA3, and HIS3. |
| Claim: | 13. The DNA expression vector of claim 11 further comprising a S. cerevisiae origin of replication. |
| Claim: | 14. The DNA expression vector of claim 13 wherein the S. cerevisiae origin of replication is based on the S. cerevisiae 2 micron DNA sequence. |
| Claim: | 15. The DNA expression vector of claim 8 further comprising a bacterial origin of replication. |
| Claim: | 16. A yeast strain comprising a DNA expression vector wherein the DNA expression vector comprises, a synthetic gene control region which comprises a sequence selected from the group consisting of SEQ ID NO:21, 22, 23, 24, 25, and 26, a a coding sequence encoding a protein, polypeptide, or peptide under the control of the control region, and an yeast selection marker. |
| Claim: | 17. A method for producing a recombinant protein, polypeptide, or peptide comprising expressing the coding sequence of the yeast strain of claim 16 . |
| Claim: | 18. The control region of claim 1 , wherein the promoter is SEQ ID NO:1. |
| Current U.S. Class: | 435/691 |
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| Primary Examiner: | Ketter, Jim |
| Attorney, Agent or Firm: | Finnegan, Alysia A. Heber, Sheldon O. |
| رقم الانضمام: | edspgr.08026082 |
| قاعدة البيانات: | USPTO Patent Grants |
| الوصف غير متاح. |