Patent
Marker proteins for diagnosing liver disease and method of diagnosing liver disease using the same
| العنوان: | Marker proteins for diagnosing liver disease and method of diagnosing liver disease using the same |
|---|---|
| Patent Number: | 7,781,180 |
| تاريخ النشر: | August 24, 2010 |
| Appl. No: | 12/379904 |
| Application Filed: | March 04, 2009 |
| مستخلص: | Using the protein chip technology, biological samples such as sera are subjected to proteome analysis. Thus, a protein which is a human fibrinogen α-E chain decomposition product and has a molecular weight of 5,900, a protein which is an apolipoprotein AII decomposition product and has a molecular weight of 7,800, and a protein which is an apolipoprotein AI decomposition product and has a molecular weight of 28,000, each showing an increase or a decrease with the habit of drinking, are newly found out. By detecting or quantifying these proteins, a liver disease in a subject such as one having a problem of drinking can be diagnosed at the early stage. |
| Inventors: | Nomura, Fumio (Chiba, JP); Sogawa, Kazuyuki (Chiba, JP); Tomonaga, Takeshi (Chiba, JP); Ochiai, Takenori (Chiba, JP); Shimada, Hideaki (Chiba, JP); Ohashi, Tatsuya (Chiba, JP); Katayama, Katsuhiro (Chiba, JP) |
| Assignees: | Nitto Boseki Co., Ltd. (Fukushima-shi, JP) |
| Claim: | 1. A method for diagnosing the probability of the onset of a liver disease, the liver disease or the prognosis of the liver disease by detecting or quantifying a marker protein for diagnosing liver disease in a sample obtained from a patient who is suspected to have the liver disease said marker protein being a 5.9 kDa protein having the amino acid sequence shown as SEQ ID NO: 1 in the sequence listing. |
| Claim: | 2. A diagnosis method according to claim 1 , wherein the liver disease is a liver disease caused by drinking alcohol. |
| Claim: | 3. A diagnosis method according to claim 1 , wherein the detection or quantification of the marker protein for diagnosing liver disease in the sample is carried out by mass spectrometry. |
| Claim: | 4. A diagnosis method according to claim 3 , wherein the diagnosis is carried out by analyzing the pattern of a spectrum obtained with a mass spectrometer. |
| Claim: | 5. A diagnosis method according to claim 4 , wherein the mass spectrometry is carried out with a laser desorption/ionization-time of flight-mass spectrometer (LDI-TOF MS). |
| Claim: | 6. A diagnosis method according to claim 5 , wherein the laser desorption/ionization-time of flight-mass spectrometer is a surface enhanced laser desorption/ionization-time of flight-mass spectrometer (SELDI-TOF MS). |
| Claim: | 7. A diagnosis method according to claim 1 , wherein the detection or quantification of the marker protein for diagnosing liver disease in the sample is carried out by an immunoassay method using an antibody against said protein. |
| Claim: | 8. A diagnosis method according to claim 7 , wherein the immunoassay method is an enzyme immunoassay method (EIA method), an immunoturbidimetry method (TIA method), a latex immuno-agglutination method (LATEX method), an electrochemiluminescence method or a fluorescence method. |
| Claim: | 9. A diagnosis method according to claim 8 , wherein the immunoassay method is an enzyme immunoassay method (EIA method). |
| Current U.S. Class: | 435/71 |
| Patent References Cited: | 5639940 June 1997 Garner et al. 1582873 October 2005 63-237795 October 1988 WO 94/16085 July 1994 WO 01/86304 November 2001 |
| Other References: | Wells, Biochemistry, vol. 29, pp. 8509-8517, 1990. cited by examiner Seffernick et al., J. Bacteriology, vol. 138, pp. 2405-2410, 2001. cited by examiner Bedossa et al., “Apolipoprotein Al is a Serum and Tissue Marker of Liver Fibrosis in Alcoholic Patients,” XP 009068725, Alcoholism, Clinical and Experimental Research, vol. 13, No. 6 (Nov./Dec. 1989), pp. 829-833. cited by examiner Pilette et al., “Histopathological evaluation of liver fibrosis: quantitative image analysis vs semi-quantitative scores, Comparison with Serum markers,” XP 002388410, J. Hepatology, 28 (1998), pp. 439-446. cited by examiner European Search Report dated Jul. 21, 2006. cited by examiner F. Nomura, et al., “Protein Chip Technology ni yoru Aratana Inshu Marker no Tansaku,” Alcohol to Igaku Seibutsugaku, vol. 23, Sep. 20, 2003, pp. 11-14. cited by other T. Poynard, et al., “A Simple Biological Index for Detection of Alcoholic Liver Disease in Drinkers,” Gastroenterology, vol. 100, No. 5 (Pt. 1), 1991, pp. 1397-1402. cited by other Wibur, et al., “Rapid similarity searches of nucleic acid and protein data banks,” Proc. Natl. Acad. Sci. USA, vol. 80 (Feb. 1983), pp. 726-730. cited by other Kohler et al., “Continuous cultures of fused cells secreting antibody of predefined specificity,” Nature, vol. 256 (Aug. 7, 1975), pp. 495-497. cited by other International Search Report dated Mar. 23, 2004. cited by other |
| Primary Examiner: | Noakes, Suzanne M. |
| Attorney, Agent or Firm: | Kratz, Quintos & Hanson, LLP |
| رقم الانضمام: | edspgr.07781180 |
| قاعدة البيانات: | USPTO Patent Grants |
| الوصف غير متاح. |