Patent
Humanized immunoglobulin loci
| العنوان: | Humanized immunoglobulin loci |
|---|---|
| Patent Number: | 7,585,668 |
| تاريخ النشر: | September 08, 2009 |
| Appl. No: | 10/893483 |
| Application Filed: | July 15, 2004 |
| مستخلص: | The present invention concerns methods and means to produce humanized antibodies from transgenic non-human animals. The invention specifically relates to novel immunoglobulin heavy and light chain constructs, recombination and transgenic vectors useful in making transgenic non-human animals expressing humanized antibodies, transgenic animals, and humanized immunoglobulin preparations. |
| Inventors: | Buelow, Roland (Palo Alto, CA, US); van Schooten, Wim (Sunnyvale, CA, US); Platzer, Josef (Geretsried, DE) |
| Assignees: | Therapeutic Human Polyclonals, Inc. (Mountain View, CA, US) |
| Claim: | 1. An isolated nucleic acid molecule comprising two or more identical units consisting of, from 5′ to 3′ direction, a 5′ nucleotide sequence, a human immunoglobulin heavy chain V gene segment, and a 3′ nucleotide sequence, wherein said 5′ nucleotide sequence and said 3′ nucleotide sequence are identical or different, and comprise 20 to 1000 contiguous nucleotides of a spacer sequence from an immunoglobulin heavy chain gene of an animal generating antibody diversity primarily by gene conversion and/or hypermutation, wherein said spacer sequence is SEQ ID NO: 35, and said animal is a rabbit. |
| Claim: | 2. The isolated nucleic acid molecule of claim 1 wherein said human immunoglobulin heavy chain V gene segment is a member of the VH3, VH1, VH5, or VH4 family. |
| Claim: | 3. A recombination vector comprising a nucleic acid molecule according to claim 1 . |
| Current U.S. Class: | 4353/201 |
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| Primary Examiner: | Wehbe, Anne Marie S |
| Attorney, Agent or Firm: | Dreger, Ginger R. Goodwin Procter LLP |
| رقم الانضمام: | edspgr.07585668 |
| قاعدة البيانات: | USPTO Patent Grants |
| الوصف غير متاح. |