Patent
Nucleic acids encoding fibroblast growth factor receptor-like proteins and uses thereof
| العنوان: | Nucleic acids encoding fibroblast growth factor receptor-like proteins and uses thereof |
|---|---|
| Patent Number: | 7,348,162 |
| تاريخ النشر: | March 25, 2008 |
| Appl. No: | 09/815108 |
| Application Filed: | March 22, 2001 |
| مستخلص: | The present invention provides Fibroblast Growth Factor Receptor-Like (FGFR-L) polypeptides and nucleic acid molecules encoding the same. The invention also provides selective binding agents, vectors, host cells, and methods for producing FGFR-L polypeptides. The invention further provides pharmaceutical compositions and methods for the diagnosis, treatment, amelioration, and/or prevention of diseases, disorders, and conditions associated with FGFR-L polypeptides. |
| Inventors: | Saris, Christiaan M. (Newbury Park, CA, US); Mu, Sharon X. (Thousand Oaks, CA, US); Xia, Min (Newbury Park, CA, US); Boone, Thomas Charles (Newbury Park, CA, US); Covey, Todd (Moorpark, CA, US) |
| Assignees: | Amgen Inc. (Thousand Oaks, CA, US) |
| Claim: | 1. An isolated nucleic acid molecule comprising a nucleotide sequence: (a) as set forth in SEQ ID NO: 1; (b) of the DNA insert in ATCC Deposit No. PTA-1062; (c) encoding the polypeptide as set forth in SEQ ID NO: 2; or (d) that hybridizes to the complement of the nucleotide sequence of (c) at 65° C. in a hybridization and wash buffer comprising 0.015 M sodium chloride and 0.0015 M sodium citrate or at 42° C. in a hybridization and wash buffer comprising 0.015 M sodium chloride, 0.0015 M sodium citrate, and 50% formamide, and encoding a polypeptide that inhibits FGF-mediated growth in bone marrow stromal cells; or (e) that is complementary to the nucleotide sequence of any of (a)-(d). |
| Claim: | 2. An isolated nucleic acid molecule comprising: (a) a region of the nucleotide sequence of SEQ ID NO: 1 or the DNA insert in ATCC Deposit No. PTA 1062 encoding a polypeptide fragment of at least 25 amino acid residues of SEQ ID NO: 2; (b) a region of the nucleotide sequence of SEQ ID NO: 1 or the DNA insert in ATCC Deposit No. PTA-1062 comprising a fragment of at least 75 nucleotides, wherein the fragment encodes a portion of the polypeptide as set forth in SEQ ID NO: 2; (c) a nucleotide sequence that hybridizes to the complement of the nucleotide sequence of either (a) or (b) at 65° C. in a hybridization and wash buffer comprising 0.015 M sodium chloride and 0.0015 M sodium citrate or at 42° C. in a hybridization and wash buffer comprising 0.015 M sodium chloride, 0.0015 M sodium citrate, and 50% formamide, and encoding a polypeptide that inhibits FGF-mediated growth in bone marrow stromal cells; or (d) a nucleotide sequence that is complementary to the nucleotide sequence of any of (a)-(c). |
| Claim: | 3. An isolated nucleic acid molecule comprising a nucleotide sequence: (a) encoding a polypeptide as set forth in SEQ ID NO: 2 which has a C- and/or N-terminal truncation, wherein the polypeptide having a C- and/or N-terminal truncation inhibits FGF-mediated growth in bone marrow stromal cells; (b) that hybridizes to the complement of the nucleotide sequence of (a) at 65° C. in a hybridization and wash buffer comprising 0.015 M sodium chloride and 0.0015 M sodium citrate or at 42° C. in a hybridization and wash buffer comprising 0:015 M sodium chloride, 0.0015 M sodium citrate, and 50% formamide, and encoding a polypeptide that inhibits FGF-mediated growth in bone marrow stromal cells; or (c) that is complementary to the nucleotide sequence of either (a) or (b). |
| Claim: | 4. A vector comprising the nucleic acid molecule of any of claims 1 , 2 , or 3 . |
| Claim: | 5. An isolated host cell comprising the vector of claim 4 . |
| Claim: | 6. The isolated host cell of claim 5 that is a eukaryotic cell. |
| Claim: | 7. The isolated host cell of claim 5 that is a prokaryotic cell. |
| Claim: | 8. A process of producing a polypeptide encoded by the nucleic acid molecule of any of claims 1 (a), 1 (b), 1 (c), or 1 (d), comprising culturing a host cell comprising the nucleic acidmolecule of any of claims 1 (a), 1 (b), 1 (c), or 1 (d) under suitable conditions to express the polypeptide, and optionally isolating the polypeptide from the culture. |
| Claim: | 9. A process of producing a polypeptide encoded by the nucleic acid molecule of any of claims 2 (a), 2 (b), or 2 (c), comprising culturing a host cell comprising the nucleic acid molecule of any of claims 2 (a), 2 (b), or 2 (c) under suitable conditions to express the polypeptide, and optionally isolating the polypeptide from the culture. |
| Claim: | 10. A process of producing a polypeptide encoded by the nucleic acid molecule of either claim 3 (a) or 3 (b), comprising culturing a host cell comprising the nucleic acid molecule of either claim 3 (a) or 3 (b) under suitable conditions to express the polypeptide, and optionally isolating the polypeptide from the culture. |
| Claim: | 11. The process of any of claims 8 , 9 , or 10 , wherein the nucleic acid molecule comprises promoter DNA other than the promoter DNA for the native Fibroblast Growth Factor Receptor-Like gene operatively linked to the nucleic acid molecule. |
| Current U.S. Class: | 435/691 |
| Patent References Cited: | 6242419 June 2001 Strachan et al. WO 99/63088 December 1999 WO 00/24756 May 2000 WO 00/58463 October 2000 |
| Other References: | Ngo et al., 1994, Computational Complexity, Protein Structure prediction, and the Levinthal Paradox, The Protein Folding Problem and Tertiary Structure Prediction, pp. 492-495. cited by examiner Wells, Aditivity of Mutational Effects in Proteins, 1990, Biochemistry, vol. 26, No. 37, pp. 8509-8517. cited by examiner Wiedemann et al., “Characterization of a Novel Protein (FGFR1) from Human Carilage Related to FGF Receptors.” Genomics, 2000, 69:275-279. cited by other Wiedemann et al., “The Mouse Fgfr;1 Gene Coding for a Novel FGF Receptor-Like Protein,” Biochemica et Biophysica Acta, 2001, 1520:247-250. cited by other Sleeman et al., “Identification of a New Fibroblast Growth Factor Receptor, FGFR5,” Gene, 2001, 271:171-182. cited by other GENEBANK Acc. No. AI 245701. cited by other Kim et al., A Novel Fibroblast Growth Factor Receptor-5 Preferentially Expressed in the Pancreas., Biochim. Biophys. Acta, 2001 1518:152-156. cited by other |
| Assistant Examiner: | Seharaseyon, Jegatheesan |
| Primary Examiner: | Saoud, Christine J. |
| Attorney, Agent or Firm: | McDonnell Boehnen Hulbert & Berghoff LLP |
| رقم الانضمام: | edspgr.07348162 |
| قاعدة البيانات: | USPTO Patent Grants |
| الوصف غير متاح. |