Patent
Cytoplasmic transduction peptides and uses thereof
| العنوان: | Cytoplasmic transduction peptides and uses thereof |
|---|---|
| Patent Number: | 7,101,844 |
| تاريخ النشر: | September 05, 2006 |
| Appl. No: | 10/509620 |
| Application Filed: | March 28, 2003 |
| مستخلص: | The present invention relates to a cytoplasmic transduction peptide (CTP) showing transduction potential, as well as cytoplasmic remaining potential and various uses thereof. The CTP of this invention exhibits a transduction potential identical or higher than the conventional protein transduction, PTD, and a strong tendency to remain in the cytoplasm, so that it is very useful in inducing cytotoxic T lymphocytes (CTL) and a drug delivery system (DDS) targeting cytoplasm. |
| Inventors: | Kim, Dae-You (Daejon Metropolitan, KR); Oh, Hae-Keun (Daejeon Metropolitan, KR); Kim, Chang-Hyun (Daejeon Metropolitan, KR); Kim, Jung-Hwan (Daejeon Metropolitan, KR); Jeon, Choon-Ju (Daejeon Metropolitan, KR); Kim, Ki-Tae (Daejeon Metropolitan, KR); Bae, Yong-Soo (Daejeon Metropolitan, KR); Choi, In-Soo (Sewon-shi, KR) |
| Assignees: | Creagene, Inc. (Daejon Metropolitan, KR) |
| Claim: | 1. A cytoplasmic transduction peptide characterized in that said cytoplasmic transduction peptide has a cell membrane transduction potential; wherein when a cell is treated with said cytoplasmic transduction peptide for a period of time and then treated with a protease, a cell membrane transduction by said cytoplasmic transduction peptide continues to occur; and after said cell membrane transduction, said cytoplasmic transduction peptide remains in the cytoplasm of said cell, wherein said cytoplasmic transduction peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:1, 2 and 13. |
| Claim: | 2. The cytoplasmic transduction peptide according to claim 1 , wherein said cytoplasmic transduction peptide comprises the amino acid sequence of SEQ ID NO:1. |
| Claim: | 3. A nucleic acid molecule encoding the cytoplasmic transduction peptide according to claim 1 . |
| Claim: | 4. A cytoplasmic transduction system comprising the cytoplasmic transduction peptide according to claim 1 and a biologically active molecule covalently linked to said peptide. |
| Claim: | 5. A method for delivering a biologically active molecule into a cytoplasm of a cell, which comprises contacting the cytoplasmic transduction system according to claim 4 to said cell. |
| Claim: | 6. A method for delivering a biologically active molecule into a cytoplasm of cells of an individual, which comprises administering the cytoplasmic transduction system according to claim 4 to said individual. |
| Claim: | 7. The method according to claim 6 , wherein said cell is liver cell or lymphoid cell. |
| Current U.S. Class: | 514/2 |
| Patent References Cited: | 5807746 September 1998 Lin et al. 6043339 March 2000 Lin et al. 95/28494 October 1995 95/34665 December 1995 00/34308 June 2000 |
| Other References: | Wouters-Tyrou, et al., (J. Biol. Chem. 1991, 266, 17388-95. cited by examiner Wender et al; “The Design, Synthesis, and Evaluation of Molecules that Enable or Enhance Cellular Uptake: Peptoid Molecular Transporters”; ;PNAS, Nov. 2000, vol. 97, No. 24, pp. 13003-13008. cited by other Schwarze et al; “In Vivo Protein Transduction: Delivery of a Biologically Active Protein into the Mouse”; Science, vol. 285, Sep. 3, 1999, pp. 1569-1572. cited by other Ho et al; “Synthetic Protein Transduction Domains: Enhanced Transduction Potential in Vitro and in Vivo”; Cancer Research 61, Jan. 15, 2001, pp. 474-477. cited by other Ford et al; “Protein Transduction: an Alterative to Genetic Invention?”; Gene Ther., Jan. 2001, vol. 8, No. 1, pp. 1-4. cited by other Vocero-Akbani et al; “Transduction of Full-Length Tat Fusion Proteins Directly into Mammalian Cells: Analysis of T Cell Receptor Activation-Induced Cell Death”; Methods Enzymol., 2000, vol. 322, pp. 508-521. cited by other |
| Assistant Examiner: | Gudibande, Satyanarayana R. |
| Primary Examiner: | Campell, Bruce R. |
| Attorney, Agent or Firm: | Nixon & Vanderhye P.C. |
| رقم الانضمام: | edspgr.07101844 |
| قاعدة البيانات: | USPTO Patent Grants |
| الوصف غير متاح. |