Patent
Tumour suppressor gene
| العنوان: | Tumour suppressor gene |
|---|---|
| Patent Number: | 6,307,036 |
| تاريخ النشر: | October 23, 2001 |
| Appl. No: | 09/257,580 |
| Application Filed: | February 25, 1999 |
| مستخلص: | Isolated DNA coding for canine p53 protein is described, along with vectors and host cells containing such isolated DNA. Methods for producing canine p53 protein by culturing host cells that contain such DNA are also described. |
| Inventors: | Milner, Jo (York, GBX); Veldhoen, Nik (York, GBX) |
| Assignees: | Yorkshire Cancer Research (Harrogate, GBX) |
| Claim: | What is claimed is |
| Claim: | 1. An isolated DNA coding for the canine p53 protein encoded by the DNA sequence given as SEQ ID NO:1. |
| Claim: | 2. An isolated DNA sequence according to claim 1 having the DNA sequence given as SEQ ID NO:1. |
| Claim: | 3. A replicative cloning vector comprising an isolated DNA according to claim 1 and a replicon operative in a host cell for said vector. |
| Claim: | 4. A host cell containing a vector according to claim 3. |
| Claim: | 5. An expression vector comprising an isolated DNA according to claim 1 operably linked to a promoter sequence that directs expression of said isolated DNA in host cells for said vector. |
| Claim: | 6. A host cell containing a vector according to claim 5. |
| Claim: | 7. A method of producing a canine p53 protein comprising culturing host cells according to claim 6 under conditions suitable for production of the protein encoded by said isolated DNA, and then recovering said protein. |
| Current U.S. Class: | 536/231; 4353/201; 4352/533; 435/691 |
| Current International Class: | C07H 2102; C12N 1500; C12N 120; C12P 2106 |
| Other References: | Leeuwen et al (Anticancer Res., 16:3737-3744), 1996.* Kraegel et al (Cancer Lett., 92:181-186), 1996.* Veldhoen et al.; Mutations of the p53 Gene in Canine Lymphoma and Evidence for Germ Line p53 Mutations in the Dog, Oncogene, 16:249-255 (1998). Veldhoen et al.; Isolation of Canine p53 cDNA and Detailed Characterization of the Full Length Canine p53 Protein, Oncogene, 16:1077-1084 (1998). |
| Primary Examiner: | Ungar, Susan |
| Attorney, Agent or Firm: | Myers Bigel Sibley & Sajovec |
| رقم الانضمام: | edspgr.06307036 |
| قاعدة البيانات: | USPTO Patent Grants |
| الوصف غير متاح. |