Patent
Geranylgeranyl diphosphate synthase and DNA coding therefor
| العنوان: | Geranylgeranyl diphosphate synthase and DNA coding therefor |
|---|---|
| Patent Number: | 5,773,273 |
| تاريخ النشر: | June 30, 1998 |
| Appl. No: | 08/410,167 |
| Application Filed: | March 24, 1995 |
| مستخلص: | DNA coding for thermostable geranylgeranyl diphosphate (GGDP) synthase derived from Sulfolobus acidocaldarius is provided. The DNA is useful for production of GGDP synthase, which is, in turn, useful for production of GGDP. |
| Inventors: | Nishino, Tokuzo (Sendai, JPX); Ohnuma, Shinichi (Sendai, JPX); Suzuki, Manabu (Sendai, JPX); Ohto, Chikara (Toyota, JPX); Asada, Chika (Toyota, JPX); Higuchi, Yuka (Toyota, JPX); Takeuchi, Yoshie (Toyota, JPX) |
| Assignees: | Toyota Jidosha Kabushiki Kaisha (Toyota, JPX) |
| Claim: | We claim |
| Claim: | 1. An isolated DNA comprising a nucleotide sequence encoding geranylgeranyl diphosphate synthase of Sulfolobus acidocaldarius. |
| Claim: | 2. The DNA according to claim 1, wherein the nucleotide sequence encodes an amino acid sequence of SEQ ID NO: 4. |
| Claim: | 3. DNA according to claim 1, wherein the nucleotide sequence comprises SEQ ID NO: 1. |
| Claim: | 4. An isolated DNA comprising a nucleotide sequence encoding a modified geranylgeranyl diphosphate synthase derived from a geranylgeranyl diphosphate synthase endogenous to Sulfolobus acidocaldarius and comprising one or more insertions, deletions or substitutions of a codon from a non-essential region of said endogenous geranylgeranyl diphosphate synthase, wherein said modified geranylgeranyl diphosphate synthase has the enzymatic activity of a geranylgeranyl diphosphate synthase endogenous to S. acidocaldarius. |
| Claim: | 5. The DNA of claim 4 wherein the nucleotide sequence encodes a modified geranylgeranyl diphosphate synthase comprising an amino acid sequence having at least 90% identity to SEQ ID NO: 4. |
| Claim: | 6. The DNA of claim 4 wherein the nucleotide sequence encodes a modified geranylgeranyl diphosphate synthase comprising an amino acid sequence having at least 95% identity to SEQ ID NO: 4. |
| Claim: | 7. The DNA of claim 4 wherein the nucleotide sequence encodes a modified geranylgeranyl diphosphate synthase having an amino acid sequence that differs from the amino acid sequence in SEQ ID NO: 4 at position 247 or 275 or at both positions 247 and 275. |
| Claim: | 8. A recombinant vector comprising DNA according to claim 1 and an additional nucleotide sequence for regulating the expression of said DNA. |
| Claim: | 9. A recombinant vector comprising DNA according to claim 1 wherein the DNA further comprises a nucleotide sequence encoding a fusion partner and an additional nucleotide sequence for regulating the expression of said DNA. |
| Claim: | 10. A recombinant vector comprising DNA according to claim 9 wherein the fusion partner is selected from the group consisting of a maltose binding protein and a glutathione-S-transferase protein. |
| Claim: | 11. A recombinant microbial cell which is transformed with a recombinant vector according to claim 8. |
| Claim: | 12. A recombinant microbial cell according to claim 11, wherein the host belongs to the genus Escherichia. |
| Claim: | 13. A recombinant microbial cell which is transformed with a recombinant vector according to claim 9. |
| Claim: | 14. A recombinant microbial cell which is transformed with a recombinant vector according to claim 10. |
| Claim: | 15. A process for production of geranylgeranyl diphosphate synthase comprising the steps of culturing the recombinant microbial cell according to claim 11 in a medium, and recovering geranylgeranyl diphosphate synthase. |
| Claim: | 16. A process for production of geranylgeranyl diphosphate synthase comprising the steps of culturing the recombinant microbial cell according to claim 13 in a medium, and recovering a geranylgeranyl diphosphate synthase fusion protein encoding a enzymatically active geranylgeranyl diphosphate synthase. |
| Claim: | 17. A process for production of geranylgeranyl diphosphate synthase comprising the steps of culturing the recombinant microbial cell according to claim 13 in a medium, obtaining a geranylgeranyl diphosphate synthase fusion protein, and liberating a enzymatically active geranylgeranyl diphosphate synthase from the fusion protein. |
| Claim: | 18. A process for production of geranylgeranyl diphosphate synthase comprising the steps of culturing the recombinant microbial cell according to claim 14 in a medium, and recovering a geranylgeranyl diphosphate synthase fusion protein encoding a enzymatically active geranylgeranyl diphosphate synthase. |
| Claim: | 19. A process for production of geranylgeranyl diphosphate comprising reacting a culture of the recombinant microbial cell according to claim 11 or geranylgeranyl diphosphate synthase isolated from said culture with a substrate selected from the group consisting of isopentyl diphosphate, dimethylallyl diphosphate, geranyl diphosphate and farnesyl diphosphate. |
| Claim: | 20. A process for the production of geranylgeranyl diphosphate comprising reacting a geranylgeranyl diphosphate synthase fusion protein obtained by a process according to claim 16 with a substrate selected from the group consisting of isopentyl diphosphate, dimethylallyl diphosphate, geranyl diphosphate and farnesyl diphosphate. |
| Claim: | 21. A process for production of geranylgeranyl diphosphate comprising reacting a culture of the recombinant microbial cell according to claim 14 or a geranylgeranyl diphosphate synthase fusion protein isolated from said culture with a substrate selected from the group comprising isopentyl diphosphate, dimethylallyl diphosphate, geranyl diphosphate and farnesyl diphosphate. |
| Current U.S. Class: | 435/193; 435/691; 435/697; 435/131; 4352/523; 43525/233; 4353/201; 536/232; 536/234 |
| Current International Class: | C12N 910; C12N 1554; C12N 1570; C12P 704 |
| Patent References Cited: | 4652639 March 1987 Stabinsky |
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| Primary Examiner: | Wax, Robert A. |
| Assistant Examiner: | Moore, William W. |
| Attorney, Agent or Firm: | Kenyon & Kenyon |
| رقم الانضمام: | edspgr.05773273 |
| قاعدة البيانات: | USPTO Patent Grants |
| الوصف غير متاح. |