Patent
Pseudorabies virus protein
| العنوان: | Pseudorabies virus protein |
|---|---|
| Patent Number: | 5,352,575 |
| تاريخ النشر: | October 04, 1994 |
| Appl. No: | 07/513,282 |
| Application Filed: | April 20, 1990 |
| مستخلص: | The present invention provides recombinant DNA molecules comprising a sequence encoding a pseudorabies virus (PRV) glycoprotein selected from the group consisting of gI, gp50, and gp63, host cells transformed by said recombinant DNA molecule sequences, the gI, gp50 and gp63 polypeptides. The present invention also provides subunit vaccines for PRV, methods for protecting animals against PRV infection and methods for distinguishing between infected and vaccinated animals. |
| Inventors: | Petrovskis, Erik A. (Kalamazoo, MI); Post, Leonard E. (Kalamazoo, MI); Timmins, James G. (Naperville, IL) |
| Assignees: | The Upjohn Company (Kalamazoo, MI) |
| Claim: | We claim |
| Claim: | 1. A method for distinguishing an animal vaccinated with a pseudorabies virus (PRV) vaccine lacking glycoprotein gI or gp63 from an animal infected with a virulent wild-type pseudorabies virus without sacrificing the animal comprising: contacting serum from the animal with PRV glycoprotein gI or gp63, and detecting the presence or absence of antibodies which specifically bind to PRV glycoprotein gI or gp63 wherein the presence of antibodies indicates that the animal has been infected with a virulent wild-type pseudorabies virus. |
| Claim: | 2. The method of claim 1 wherein the animal has been vaccinated with a PRV vaccine lacking glycoprotein gI and the serum is contacted with PRV glycoprotein gI. |
| Claim: | 3. The method of claim 1 wherein the presence or absence of the antibodies is detected using an ELISA. |
| Claim: | 4. A mercantile kit useful in performing the method of claim 1, comprising multiple containers wherein one of said containers has therein PRV glycoprotein gI or gp63 and another of said containers has therein a reagent for detection of antibodies which specifically bind to gI or gp63. |
| Claim: | 5. The mercantile kit of claim 4 wherein the PRV glycoprotein is gI. |
| Current U.S. Class: | 435/5; 435/792; 435/794; 435/975 |
| Current International Class: | G01N 33569 |
| Patent References Cited: | 4514497 April 1985 Kit et al. 4609548 September 1986 Kit et al. 4680176 July 1987 Berns et al. 4703011 October 1987 Kit et al. 4711850 December 1987 Kit et al. 4810634 March 1989 Post et al. 5047237 September 1991 Cochran et al. |
| Other References: | Stevely, J. Virol. 16(5):944-950 (Oct. 1975). Todd et al., "Enzyme-linked immunosorbent assay for detecting antibodies to Aujeszky's disease virus in pigs", Vet. Record, 109:534-537 (Dec. 1981). D. P. Gustafson, "Pseudorabies," in Diseases of Swine, 5th ed., A. D. Leman et al., 209-23 (1981). Jones, T. C. and R. D. Hunt, "Pseudorabies (Infectious Bulbar Paralysis, Aujeszky's Disease, Mad Itch) in Veterinary Pathology", 5th Ed., Lea & Febiger, 322-26 (1983). C. E. Aronson, "Pseudo-Cine.TM.with Havlogen.RTM.," Veterinary Pharmaceuticals & Biologicals 15/90 (1982-1983). C. E. Aronson, "PR-VAC.RTM.," and PR-VAC.RTM.-Killed, and PR-VAC.RTM./Leptoferm-5, Veterinary Pharmaceuticals & Biologicals 15/98-99 (1982-1983). Ben-Porat, T. and A. S. Kaplan, "Synthesis of Proteins in Cells Infected with Herpesvirus V. Viral Glycoproteins," Virology 41:265-73 (1970). Kaplan, A. S. and T. Ben-Porat, "Synthesis of Proteins in Cells Infected with Herpesvirus, VI. Characterization of the Proteins of the Viral Membrane," Proc. of Natn'l Acad. of Sciences 66(3):799-806. Norid, B. and B. F. Vestergaard, "Immunoelectrophoretic Identification and Purification of Herpes Simplex Virus Antigens Released from Infected Cells in Tissue Culture," Intervirology 11:104-10 (1979). Khristova, V. et al., "Thymidine Kinase Activity of Virulent and Vaccinal Strains of Aujezsky's Disease Virus," Veterinary Science XXII(3):15-22. G. Tatarov, "Apathogenic Mutant of the Aujeszky Virus Induced by 5-Iodo-2-Deoxyuridine (IUDR)," Zentralblatt Veterinarmedizin 15:847-53 (1968). Randall, R. E. et al., "Glycoproteins with Type Common and Type Specific Antigenic Sites Excreated from Cells Infected with Herpes Simplex Virus Types 1 and 2," J. Gen. Virol. 48:297-310 (1980). Van Zaane, D. et al., "Molecular-Biological Characterization of Marek's Disease Virus," Virology 121:116-32 (1982). Randall, R. E. and R. W. Honess, "Proteins of Herpesvirus Saimiri: Identification of Two Virus Polypeptides Released into the Culture Medium of Productively Infected Cells," J. Gen. Virol. 51:445-49 (1980). Gielkens, A. L. J. et al., "Genome Differences Among Field Isolates and Vaccine Strains of Pseudorabies Virus," J. Gen. Virol. 66:69-82 (1985). Valenzuela, P. et al., "Antigen Engineering in Yeast: Synthesis and Assembly of Hybrid Hepatitis B Surface Antigen-Herpes Simplex 1 gD Particles," Bio/Technology 3:323-26 (1985). Kieny, M. P. et al., "Expression of Rabies Virus Glycoprotein From a Recombinant Vaccinia Virus," Nature 312:163-66 (1984). Holland, T. C. et al., "Antigenic Variants of Herpes Simplex Virus Selected with Glycoprotein-Specific Monoclonal Antibodies," J. of Virology 45(2):672-82 (1983). Post, L. E. and B. Roizman, "A Generalized Technique for Deletion of Specific Genes in Large Genomes: .alpha. Gene 22 of Herpes Simplex Virus 1 is Not Essential for Growth," Cell 25:227-32 (1981). Todd, D. and J. B. McFerran, "Control of Aujeszky's Disease," The Veterinary Record 117(24):647(1985). Thomsen, D. R. et al., "Replication and Virulence of Pseudorabies Virus Mutants Lacking Glycoprotein gX," J. of Virology 61(1):229-32 (1987). Sandri-Goldin, R. M. et al., "Method for Induction of Mutations in Physically Defined Regions of the Herpes Simplex Virus Genome," J. of Virology 38(1):41-49 (1981). Quint, W. et al., "Construction and Characterization of Deletion Mutanta of Pseudorabies Virus: A New Generation of `Live` Vaccines," J. Gen. Virol. 68:523-34 (1987). Roizman et al., Science, 229, 1208-14 (Sep. 1985). Marchioli et al., Am J. Vet. Res., 481(11), 1577-83 (1987). Davison et al., Chapter 7, pp. 103-124, in Recombinant DNA Research and Viruses, Becker, et al. 1985. Thomsen et al., J. Virol, 61(1), 229-37 (1987). Petrovskis et al., J. Virol, 60(3), 1166-9 (1986). Ben-Porat, T. et al., J. Virol, 57(1), 191-6 (Jan. 1986). Robbins et al., J. Virol, 59(3) 635-45, (Sep. 1986). |
| Primary Examiner: | Kepplinger, Esther M. |
| Assistant Examiner: | Bidwell, Carol E. |
| Attorney, Agent or Firm: | Dannley, Jr., James D. Steele, Gregory W. Koivuniemi, Paul J. |
| رقم الانضمام: | edspgr.05352575 |
| قاعدة البيانات: | USPTO Patent Grants |
| الوصف غير متاح. |