التفاصيل البيبلوغرافية
| العنوان: |
DETECTION OF DYSPLASTIC OR NEOPLASTIC CELLS USING ANTI-MCM6 ANTIBODIES |
| Document Number: |
20120178103 |
| تاريخ النشر: |
July 12, 2012 |
| Appl. No: |
13/406761 |
| Application Filed: |
February 28, 2012 |
| مستخلص: |
Determination of cellular growth abnormality, particularly cancerous abnormality, by detection of target polypeptides or encoding mRNA, where the target polypeptides are members of the preinitiation complex of DNA replication in tissue, cells or fluid. Target polypeptides include CDC6, MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7. Test samples include tissue of the cervix (both biopsy and smear samples), breast, colon, lung, bladder, skin, larynx, oesophagus, bronchus, lymph nodes and urinary tract (both biopsy and cytology smear samples), in determination of cancerous and pre-cancerous cellular growth abnormality, and cells spun from urine, blood and serum, in determination of haematological malignancies and evidence of metastatic sarcoma and carcinoma. |
| Inventors: |
Laskey, Ronald A. (Cambridge, GB); Williams, Gareth H. (Cambridge, GB); Coleman, Nicholas (Cambridge, GB) |
| Assignees: |
Cancer Research Technology Limited (London, GB) |
| Claim: |
1. A method of determining the presence or absence of dysplastic or neoplastic cells in a test sample containing cells from an individual, the method comprising: contacting the test sample with an antibody or antibody fragment directed against Minichromosome Maintenance protein 3 (MCM3 protein); and determining the amount and/or pattern of binding of said antibody or antibody fragment to said test sample; whereby an increase in said amount and/or a difference in said pattern if detected for the test sample compared with a normal sample is indicative of presence of dysplastic or neoplastic cells in said test sample. |
| Claim: |
2. A method according to claim 1 wherein the test sample comprises a specimen selected from the group consisting of a sputum sample, a bronchio-alveolar lavage sample, a urine sample, a breast duct fluid sample, a brushing from the alimentary tract, a cervical cytology sample, and a fecal sample. |
| Claim: |
3. A method according to claim 1 wherein the sample is provided from fluid taken from the individual. |
| Claim: |
4. A method according to claim 3 wherein a sample of cells is provided from said fluid. |
| Claim: |
5. A method according to claim 3 wherein the fluid is urine. |
| Claim: |
6. A method according to claim 1 wherein a population of individuals is screened. |
| Claim: |
7. A method according to claim 1 wherein the sample is a cervical smear. |
| Claim: |
8. A method according to claim 7 wherein a population of individuals is screened. |
| Claim: |
9. A method according to claim 3 wherein a population of individuals is screened. |
| Claim: |
10. A method according to claim 5 wherein a population of individuals is screened. |
| Current U.S. Class: |
435/721 |
| Current International Class: |
01 |
| رقم الانضمام: |
edspap.20120178103 |
| قاعدة البيانات: |
USPTO Patent Applications |