التفاصيل البيبلوغرافية
| العنوان: |
BIOCATALYSTS AND METHODS FOR CONVERSION OF HEMICELLULOSE HYDROLYSATES TO BIOBASED PRODUCTS |
| Document Number: |
20110195464 |
| تاريخ النشر: |
August 11, 2011 |
| Appl. No: |
13/122985 |
| Application Filed: |
November 17, 2009 |
| مستخلص: |
The invention relates to processes and biocatalysts for producing ethanol and other useful products from biomass and/or other materials. Initial processing of lignocellulosic biomass frequently yields methylglucuronoxylose (MeGAX) and related products which are resistant to further processing by common biocatalysts. Strains of Enterobacter asburiae are shown to be useful in bioprocessing of MeGAX and other materials into useful bioproducts such as ethanol, acetate, lactate, and many others. Genetic engineering may be used to enhance production of desired bioproducts. |
| Inventors: |
Preston, James F. (Micanopy, FL, US) |
| Assignees: |
University of Florida Research Foundation, Inc. (Gainesville, FL, US) |
| Claim: |
1-28. (canceled) |
| Claim: |
29. An isolated strain of Enterobacter asburiae. |
| Claim: |
30. The isolated E. asburiae strain of claim 29, wherein said strain is selected from the group consisting of JDR-1, E1, and L1. |
| Claim: |
31. The isolated E. asburiae strain of claim 29, wherein said strain comprises one or more genetic modifications selected from the group consisting of: incorporation and/or overexpression of a gene encoding CRP*; incorporation and/or overexpression of a gene encoding xylose reductase; incorporation and/or overexpression of a gene encoding xylitol dehydrogenase; and inactivation of a gene encoding xylulokinase. |
| Claim: |
32. The isolated E. asburiae strain of claim 29, wherein said strain comprises one or more genetic modifications selected from the group consisting of: incorporation and/or overexpression and/or inactivation of a gene encoding L-lactate dehydrogenase; incorporation and/or overexpression and/or inactivation of a gene encoding D-lactate dehydrogenase; inactivation of a gene encoding fumarate reductase (frd); inactivation of a gene encoding alcohol/aldehyde dehydrogenase (adh); inactivation of a gene encoding pyruvate formate lyase (pfl); inactivation of a gene encoding acetate kinase (ack); and inactivation of a gene encoding methylglyoxal synthase (mgs). |
| Claim: |
33. The isolated E. asburiae strain of claim 29, wherein said strain comprises one or more genetic modifications selected from the group consisting of: insertion and/or overexpression of a gene encoding pyruvate decarboxylase; insertion and/or overexpression of a gene encoding alcohol dehydrogenase; inactivation of a gene encoding lactate dehydrogenase; inactivation of a gene encoding phosphoenolpyruvate carboxylase; inactivation of a gene encoding acetate kinase; and inactivation of a gene encoding pyruvate formate lyase. |
| Claim: |
34. The isolated E. asburiae strain of claim 29, wherein said strain comprises one or more genetic modifications selected from the group consisting of: overexpression of a gene encoding PEP carboxykinase; inactivation of a gene encoding pyruvate formate lyase; and inactivation of a PEP-dependent phosphotransferase system gene. |
| Claim: |
35. The isolated E. asburiae strain of claim 29, wherein said strain comprises one or more further genetic modifications selected from the group consisting of: inactivation of a gene encoding acetate kinase; inactivation of a gene encoding alcohol dehydrogenase; inactivation of a gene encoding aspartate aminotransferase; inactivation of a gene encoding citrate lyase; inactivation of a gene encoding lactate dehydrogenase; inactivation of a gene encoding methylglyoxal synthase; inactivation of a gene encoding pyruvate oxidase; inactivation of a gene encoding phosphate acetyltransferase; inactivation of a gene encoding malic enzyme; and inactivation of a gene encoding threonine dehydratase. |
| Claim: |
36. The isolated E. asburiae strain of claim 29, wherein said strain comprises one or more genetic modifications selected from the group consisting of: incorporation and/or overexpression of a gene encoding alanine dehydrogenase; inactivation of a gene encoding alanine racemase; inactivation of a gene encoding lactate dehydrogenase; inactivation of a gene encoding alcohol dehydrogenase; inactivation of a gene encoding fumarate reductase; inactivation of a gene encoding pyruvate formate lyase; inactivation of a gene encoding acetate kinase; and inactivation of a gene encoding methylglyoxal synthase. |
| Claim: |
37. The isolated E. asburiae strain of claim 29, wherein said strain comprises one or more genetic modifications selected from the group consisting of: incorporation and/or overexpression of a gene encoding cellobiose utilizing enzyme; incorporation and/or overexpression of a gene encoding phospho-β-glucosidase; and incorporation and/or overexpression of a gene encoding an endoglucanase or cellulase. |
| Claim: |
38. The isolated E. asburiae strain of claim 29, wherein said strain comprises one or more genetic modifications selected from the group consisting of: inactivation of a gene encoding lactate dehydrogenase; inactivation of a gene encoding pyruvate formatelyase; inactivation of a gene encoding fumarate reductase; inactivation of a gene encoding (F1F0)H+-ATP synthase; inactivation of a gene encoding alcohol/aldehyde dehydrogenase; and inactivation of a gene encoding 2-ketoglutarate dehydrogenase. |
| Claim: |
39. The isolated E. asburiae strain of claim 29, wherein said strain comprises one or more further genetic modifications selected from the group consisting of: inactivation of a gene encoding acetate kinase; and inactivation of a gene encoding pyruvate oxidase. |
| Claim: |
40. The isolated E. asburiae strain of claim 29, wherein said strain comprises one or more genetic modifications selected from the group consisting of: incorporation and/or overexpression of a gene encoding glycerol-3-phosphate dehydrogenase; incorporation and/or overexpression of a gene encoding glycerol-3-phosphatase; incorporation and/or overexpression of a gene encoding glycerol dehydratase; incorporation and/or overexpression of a gene encoding 1,3-propanediol oxidoreductase; incorporation and/or overexpression of a gene encoding aldose reductase; and incorporation and/or overexpression of a gene encoding glycerol dehydrogenase. |
| Claim: |
41. The isolated E. asburiae strain of claim 29, wherein said strain comprises one or more genetic modifications selected from the group consisting of: inactivation of a gene encoding pyruvate formate lyase; and inactivation of a gene encoding acetolactate synthase. |
| Claim: |
42. A process for fermenting MeGAX comprising: (a) forming a substrate from biomass materials; (b) subjecting the substrate to acid hydrolysis; (c) selecting and isolating a strain of Enterobacter asburiae that has the ability to ferment MeGAX, said strain of Enterobacter asburiae being, optionally, genetically modified; (d) inoculating the acid hydrolyzed substrate with the selected strain of Enterobacter asburiae to ferment MeGAX under conditions favorable for cell viability and conversion of MEGAX to a fermentation product; and (e) optionally, recovering said fermentation product. |
| Claim: |
43. The process of claim 42, wherein the Enterobacter asburiae an Enterobacter asburiae strain has been genetically modified. |
| Claim: |
44. The process of claim 42, wherein the biomass materials contain hemicellulose. |
| Claim: |
45. The process of claim 42, wherein the acid hydrolysis is dilute acid hydrolysis. |
| Claim: |
46. A process for fermenting MeGAX comprising: (a) selecting and/or isolating a strain of Enterobacter asburiae that has the ability to ferment MeGAX in a biomass, said strain of Enterobacter asburiae being, optionally, genetically modified; (b) inoculating culture media comprising MeGAX with the selected strain of Enterobacter asburiae to ferment MeGAX under conditions favorable for cell viability and conversion of MEGAX to a fermentation product; and (c) optionally, recovering fermentation product from the substrate. |
| Claim: |
47. The process of claim 46, wherein the culture media contains hemicellulose. |
| Claim: |
48. The process of claim 46, wherein said fermentation product is acetate/acetic acid; ethanol; methanol; succinate/succinic acid; lactate/lactic acid; formate/formic acid; acetate/acetic acid; 2,3-butanediol; or combinations thereof. |
| Claim: |
49. A process for fermenting a substrate comprising: (a) selecting and isolating a strain of Enterobacter asburiae that has the ability to ferment a biomass substrate, said strain of Enterobacter asburiae being, optionally, genetically modified; (b) inoculating culture media comprising said substrate with the selected strain of Enterobacter asburiae and fermenting said substrate under conditions favorable for cell viability and conversion of the substrate to a fermentation product; and (c) optionally, recovering fermentation product from the substrate. |
| Claim: |
50. The process of claim 49, wherein said fermentation product acetate/acetic acid; ethanol; methanol; succinate/succinic acid; lactate/lactic acid; formate/formic acid; acetate/acetic acid; 2,3-butanediol; or combinations thereof. |
| Claim: |
51. The process of claim 49, wherein said substrate is D-glucose, D-xylose, D-mannose, L-arabinose, D-galactose, glucuronate, or various combinations thereof. |
| Current U.S. Class: |
435/105 |
| Current International Class: |
12; 12; 12; 12; 12; 12; 12; 12; 12; 12 |
| رقم الانضمام: |
edspap.20110195464 |
| قاعدة البيانات: |
USPTO Patent Applications |