التفاصيل البيبلوغرافية
| العنوان: |
Device and method for quantitatively determining an analyte, a method for determining an effective size of a molecule, a method for attaching molecules to a substrate, and a device for detecting molecules |
| Document Number: |
20110100822 |
| تاريخ النشر: |
May 5, 2011 |
| Appl. No: |
12/923588 |
| Application Filed: |
September 29, 2010 |
| مستخلص: |
A device for quantitatively determining an analyte is provided to conspicuously improve the performance of the quantitative determination. This device is equipped with a flow channel, an analyte detecting unit for capturing and detecting the analyte, and a quantitative measurement unit for quantitatively determining the analyte, wherein a signal generated when the analyte detecting unit has detected the analyte is divided into a plurality of parts in the direction of the flow in the flow channel at the quantitative measurement unit for processing. Also provided are technologies including one for controlling the density of molecules attached to the surface of a solid. In these technologies, when molecules are attached to a substrate, the density of attached molecules is controlled, by having an electrolyte also present in a solution containing the molecules to adjust the screening effect by the electrolyte, and by taking into consideration the effective size of a molecule. |
| Inventors: |
Arinaga, Kenji (Kawasaki, JP); Rant, Ulrich (Garching, DE); Fujihara, Tsuyoshi (Kawasaki, JP); Fujita, Shozo (Kawasaki, JP) |
| Assignees: |
FUJITSU LIMITED (Kawasaki, JP) |
| Claim: |
1-29. (canceled) |
| Claim: |
30. A method for attaching molecules having an electric charge to a substrate, wherein the density of said attached molecules is controlled, by having an electrolyte also present in a solution containing said molecules to adjust the screening effect by said electrolyte, and by taking into consideration the effective size of a molecule when said molecules having an electric charge are attached to a substrate. |
| Claim: |
31. A method for attaching molecules having an electric charge to a substrate according to claim 30, wherein the effective size of a molecule having an electric charge in a solution containing the electrolyte and the molecules having an electric charge is estimated from the screening effect by said electrolyte, and the thus obtained effective size is used as said effective size. |
| Claim: |
32. A method for attaching molecules having an electric charge to a substrate according to claim 30, wherein an electrolyte comprising a monovalent cation and a monovalent anion is used as said electrolyte. |
| Claim: |
33. A method for attaching molecules having an electric charge to a substrate according to claim 32, wherein said electrolyte comprising a monovalent cation and a monovalent anion is NaCl, KCl or a mixture thereof. |
| Claim: |
34. A device for attaching molecules having an electric charge to a substrate, wherein the density of molecules attached to the substrate can be controlled, using the method for attaching molecules to a substrate according to claim 30. |
| Claim: |
35. A device for detecting molecules, wherein the method for attaching molecules to a substrate according to claim 30 is used, and a substrate of which the density of attached molecules is controlled so that the distance between adjacent molecules attached to the substrate is not less than twice the effective length of a molecule, is used as a detecting unit for said molecule. |
| Claim: |
36. A device for detecting molecules, wherein the method for attaching molecules to a substrate according to claim 30 is used, and a substrate of which the density of attached molecules is controlled so that the distance between adjacent molecules attached to the substrate is less than twice the effective length of a molecule, is used as a detecting unit for said molecule. |
| Claim: |
37. A device for detecting molecules according to claim 35, wherein said molecule having an electric charge comprises a material selected from the group consisting of proteins, DNAs, RNAs, antibodies, natural or artificial single-stranded nucleotides, natural or artificial double-stranded nucleotides, aptamers, products obtained by limited decomposition of antibodies with a protease, organic compounds having affinity to proteins, biomacromolecules having affinity to proteins, complex materials thereof, ionic polymers charged positively or negatively, and arbitrary combinations thereof. |
| Claim: |
38. A device for detecting molecules according to claim 35, wherein said molecule having an electric charge comprises a thiol group. |
| Claim: |
39. A device for detecting molecules according to claim 35, wherein said molecule having an electric charge comprises a fluorescent pigment. |
| Claim: |
40. A device for detecting molecules according to claim 35, wherein said substrate is made of an electroconductive material, a semiconducting material, or an insulating material. |
| Claim: |
41. A device for detecting molecules according to claim 40, wherein said substrate is made of gold or platinum. |
| Claim: |
42. A device for detecting molecules according to claim 36, wherein said molecule having an electric charge comprises a material selected from the group consisting of proteins, DNAs, RNAs, antibodies, natural or artificial single-stranded nucleotides, natural or artificial double-stranded nucleotides, aptamers, products obtained by limited decomposition of antibodies with protease, organic compounds having affinity of proteins, biomacromolecules having affinity to proteins, complex materials thereof, ionic polymers charged positively or negatively, and arbitrary combinations thereof. |
| Claim: |
43. A device for detecting molecules according to claim 36, wherein said molecule having an electric charge comprise a thiol group. |
| Claim: |
44. A device for detecting molecules according to claim 36, wherein said molecule having an electric charge comprises a fluorescent pigment. |
| Claim: |
45. A device for detecting molecules according to claim 36, wherein said substrate is made of an electroconductive material, a semiconducting material, or an insulating material. |
| Claim: |
46. A device for detecting molecules according to claim 45, wherein said substrate is made of gold or platinum. |
| Current U.S. Class: |
204/550 |
| Current International Class: |
01; 12 |
| رقم الانضمام: |
edspap.20110100822 |
| قاعدة البيانات: |
USPTO Patent Applications |