التفاصيل البيبلوغرافية
| العنوان: |
Automated transient image cytometry |
| Document Number: |
20100289887 |
| تاريخ النشر: |
November 18, 2010 |
| Appl. No: |
12/454217 |
| Application Filed: |
May 13, 2009 |
| مستخلص: |
A method, system, and instrument for automatically measuring transient activity in cells uses image time sequences to identify transients in cells. Preferably, the transient activity is stimulated or provoked in synchronism with acquisition of the image time sequences. A cell mask is applied to each image of an image time sequence in order to localize the transient activity with respect to each cell. Localization enables cell-by-cell analysis of properties of the transient activity. |
| Inventors: |
Charlot, David J. (La Jolla, CA, US); Ingermanson, Randall S. (Battleground, WA, US); McDonough, Patrick M. (San Diego, CA, US); Price, Jeffrey H. (Rancho Santa Fe, CA, US) |
| Assignees: |
Vala Sciences, Inc. (San Dieogo, CA, US) |
| Claim: |
1. A computer executed method for measuring transient activity in cells, comprising the computer-executed steps of: acquiring a time-series sequence of magnified images of cells exhibiting transient activity; generating a time-series average image from the sequence of magnified images; generating cell periphery masks using the time-series average image; obtaining measurements of transient activity intensities on a cell-by-cell basis by applying the cell periphery masks to the sequence of magnified images; and, generating graphical representations of the transient activity based upon the measured transient activity intensities. |
| Claim: |
2. The computer executed method of claim 1, wherein acquiring a time-series sequence of magnified images includes: autofocusing an objective; operating a camera shutter optically coupled to the objective; and then, applying a stimulation signal to the cells. |
| Claim: |
3. An instrument for obtaining a time-series sequence of magnified images of transient activity in cells, comprising: an adjustable mechanical stage to position a well of multi-well plate; means for applying a stimulus signal in the well; a microscope objective with a field of view containing the well; and, a camera optically coupled to the microscope objective. |
| Current U.S. Class: |
348/79 |
| Current International Class: |
04; 06 |
| رقم الانضمام: |
edspap.20100289887 |
| قاعدة البيانات: |
USPTO Patent Applications |