التفاصيل البيبلوغرافية
| العنوان: |
Hybridization Device and Hybridization Method |
| Document Number: |
20070238870 |
| تاريخ النشر: |
October 11, 2007 |
| Appl. No: |
11/572506 |
| Application Filed: |
July 29, 2005 |
| مستخلص: |
The hybridization device of the invention aims to attain a hybridization reaction of high reproducibility. A hybridization device 2 for a hybridization reaction of nucleic acid has a cover member 10 that defines a cavity 12, which includes a nucleic acid fixation area 6 of a substrate 4 for fixation of a nucleic acid probe and has capacity for storage of a liquid for the hybridization reaction therein. At least part of an area exposed to inside of the cavity 12 forms a hydrophobic region 18. Adequate control of the surface characteristic of the area exposed to the cavity for implementing the hybridization reaction desirably enhances the signal intensity and reduces a variation in signal intensity, thus attaining the hybridization reaction of high reproducibility. |
| Inventors: |
Kawase, Mitsuo (Aichi, JP); Yoshida, Yasuko (Aichi, JP); Yamada, Kazunari (Aichi, JP); Takase, Tomokazu (Gifu, JP) |
| Assignees: |
NGK Insulators, Ltd. (Nagoya-City, JP) |
| Claim: |
1. A hybridization device for a hybridization reaction of nucleic acid, said hybridization device comprising: a cover member that defines a cavity, which includes a nucleic acid probe fixation area of a substrate for fixation of a nucleic acid probe and has capacity for storage of a liquid for the hybridization reaction therein, wherein at least part of an area exposed to inside of the cavity forms a hydrophobic region. |
| Claim: |
2. A hybridization device according to claim 1, wherein the hydrophobic region is formed in at least part of the cover member. |
| Claim: |
3. A hybridization device according to claim 1, wherein the cover member has the hydrophobic region in an area opposed to the nucleic acid probe fixation area. |
| Claim: |
4. A hybridization device according to claim 1, wherein the hydrophobic region has a water contact angle of not less than 30 degrees. |
| Claim: |
5. A hybridization device for a hybridization reaction of nucleic acid, said hybridization device comprising: a cover member that defines a cavity, which includes a nucleic acid probe fixation area of a substrate for fixation of a nucleic acid probe and has capacity for storage of a liquid for the hybridization reaction therein, wherein an opposed area of the cover member facing the nucleic acid probe fixation area has a thickness of not less than 300 μm. |
| Claim: |
6. A hybridization device according to claim 5, wherein the cover member has a hydrophobic region formed in at least part of an area exposed to inside of the cavity. |
| Claim: |
7. A hybridization device for a hybridization reaction of nucleic acid, said hybridization device comprising: a cover member that defines a cavity, which includes a nucleic acid probe fixation area of a substrate for fixation of a nucleic acid probe and has capacity for storage of a liquid for the hybridization reaction therein, wherein the nucleic acid probe fixation area included in the cavity has a variation coefficient of spatial height of not higher than 50%. |
| Claim: |
8. A hybridization device according to claim 7, wherein an average spatial height is not less than 15 μm. |
| Claim: |
9. A hybridization device according to claim 7, wherein the cover member has a hydrophobic region formed in at least part of an area exposed to inside of the cavity. |
| Claim: |
10. A hybridization device according to claim 1, wherein the cover member has at least a sheet element and a spacer to be interposed between the sheet element and the substrate. |
| Claim: |
11. A hybridization device according to claim 1, wherein the cover member has an opening for supply of the liquid into the cavity in an area opposed to the nucleic acid probe fixation area, and the opening is configured to have a projected portion formed by outward extension of an inner circumferential wall of the cavity. |
| Claim: |
12. A hybridization device according to claim 11, wherein the cover member has multiple openings located on both ends of the cavity in a longitudinal direction. |
| Claim: |
13. A hybridization device according to claim 1, wherein an opposed area of the cover member facing the nucleic acid probe fixation area is composed of one or multiple materials selected from the group consisting of polycarbonates, polyolefins, polyamides, polyimides, acrylic resins, fluorides thereof, and poly(vinyl halides). |
| Claim: |
14. A hybridization device according to claim 1, wherein an opposed area of the cover member facing the nucleic acid probe fixation area has at least either of a concavity and a convexity. |
| Claim: |
15. A hybridization device according to claim 1, wherein the cover member is integrated with the substrate in a detachable manner and has a tab layer with a holdable, extended end from the substrate and the cover member. |
| Claim: |
16. A hybridization method of nucleic acid, said hybridization method comprising: a setting step of setting a hybridization device for a hybridization reaction of nucleic acid according to claim 1 to a substrate with a nucleic acid probe fixation area for fixation of a nucleic acid probe; and a hybridization step of implementing a hybridization reaction of an object nucleic acid, which is contained in a liquid supplied to a cavity including the nucleic acid probe fixation area, with the nucleic acid probe in the cavity. |
| Claim: |
17. A hybridization method according to claim 16, wherein the hybridization step implements the hybridization reaction while the substrate and the hybridization device stand still. |
| Claim: |
18. A hybridization method according to claim 17, wherein the hybridization step stirs the liquid in the cavity. |
| Claim: |
19. A hybridization method according to claim 18, wherein the hybridization step implements the hybridization reaction in the presence of a specific gas in the cavity. |
| Claim: |
20. A hybridization method according to claim 19, wherein the hybridization step moves the specific gas in the cavity to stir the liquid. |
| Claim: |
21. A hybridization method according to claim 18, wherein the hybridization step implements the hybridization reaction while the substrate and the hybridization device defining the cavity is moved. |
| Claim: |
22. A hybridization method of nucleic acid, said hybridization method comprising: a hybridization step of implementing a hybridization reaction of an object nucleic acid, which is contained in a liquid supplied to a cavity, with a nucleic acid probe in the cavity, where the cavity includes a nucleic acid probe fixation area of a substrate for fixation of the nucleic acid probe and has capacity for storage of the liquid for the hybridization reaction therein, wherein the hybridization step moves a specific gas present in the cavity to stir the liquid. |
| Claim: |
23. A hybridization reaction kit for a hybridization reaction of nucleic acid, said hybridization reaction kit comprising: a substrate that has a nucleic acid probe fixation area for fixation of a nucleic acid probe; and a cover member that defines a cavity, which includes the nucleic acid probe fixation area of the substrate and has capacity for storage of a liquid for the hybridization reaction therein, wherein at least part of an area exposed to inside of the cavity forms a hydrophobic region. |
| Claim: |
24. A hybridization reaction kit according to claim 23, wherein the cover member is integrated with the substrate in a detachable manner and has a tab layer with a holdable, extended end from the substrate and the cover member. |
| Claim: |
25. A nucleic acid array, comprising: a substrate that has a nucleic acid probe fixation area with at least one nucleic acid probe fixed therein; and a cover member that defines a cavity, which includes the nucleic acid probe fixation area of the substrate and has capacity for storage of a liquid for a hybridization reaction of nucleic acid therein, wherein at least part of an area exposed to inside of the cavity forms a hydrophobic region. |
| Claim: |
26. A nucleic acid array according to claim 25, wherein the cover member is detachably attached to the substrate. |
| Claim: |
27. A nucleic acid array according to claim 25, wherein the cover member is integrated with the substrate in a detachable manner, and the cover member and the substrate are laid one upon the other via a tab layer having a holdable, extended end from the substrate and the cover member. |
| Current U.S. Class: |
536025/300 |
| Current International Class: |
12 |
| رقم الانضمام: |
edspap.20070238870 |
| قاعدة البيانات: |
USPTO Patent Applications |