التفاصيل البيبلوغرافية
| العنوان: |
Specific-C-peptide assay method |
| Document Number: |
20050164303 |
| تاريخ النشر: |
July 28, 2005 |
| Appl. No: |
11/030987 |
| Application Filed: |
January 10, 2005 |
| مستخلص: |
The present invention relates to a specific C-peptide assay method which can eliminate all interference due to proinsulin and its intermediates, in particular des-31,32-proinsulin and/or des-64,65-proinsulin. It also relates to antibodies for carrying out this assay. |
| Inventors: |
Bouanani, Majida (Douglas, GB); Kamal, Nadia (Lyon, FR); Larue, Catherine Christianne Marie (Vaucresson, FR); Mani, Jean-Claude (Montpellier, FR); Pau, Bernard Christian (Montpellier, FR); Siohan, Elisabeth (Millery, FR) |
| Claim: |
1-19. (canceled) |
| Claim: |
20. An antibody specific to des-31,32-proinsulin and proinsulin which is capable of interfering with binding of an anti-C-peptide antibody and which recognizes the epitope of sequence Leu Gln Lys Arg Gly Ile Val Glu Gln (SEQ ID NO: 2). |
| Claim: |
21. The antibody according to claim 20, wherein the antibody is a monoclonal antibody produced by a hybridoma deposited at Collection Nationale de Cultures de Microorganismes at the Institut Pasteur on 4 Jun. 1997, registration number I-1873. A method for assaying C-peptide in a sample suspected of containing C-peptide, intact proinsulin, des-31,32 proinsulin, and/or des-64,65-proinsulin comprising: a) contacting the sample with at least one anti-C-peptide antibody which specifically binds an epitope present entirely in the C-peptide and a second antibody which is selected from: an anti-proinsulin antibody which is specific to intact proinsulin and des-31,32-proinsulin and recognizes an epitope located in an intact A-chain-C-peptide (AC) junction of proinsulin and des-31,32 proinsulin, said anti-proinsulin AC junction antibody epitope being either adjacent to or overlapping with the epitope of said at least one anti-C-peptide antibody, or an anti-proinsulin antibody which is specific to intact proinsulin and des-64,65-proinsulin and recognizes an epitope located in an intact B-chain-C-peptide (BC) junction of proinsulin and des-64,65-proinsulin, said anti-proinsulin BC junction antibody epitope being either adjacent to or overlapping with the epitope of said at least one anti-C-peptide antibody, or a mixture of said anti-proinsulin AC junction antibody and said anti-proinsulin BC junction antibody under conditions such that said at least one anti-C-peptide antibody binds to C-peptide in the sample, binding of the anti-proinsulin AC junction antibody to any proinsulin and/or des-31,32-proinsulin interferes with binding of said at least one anti-C-peptide antibody to intact proinsulin and des-31,32-proinsulin in the sample, and/or binding of the anti-proinsulin BC junction antibody to any proinsulin and/or des-64,65-proinsulin interferes with binding of said at least one anti-C-peptide antibody to intact proinsulin and des-64,65-proinsulin in the sample; and b) detecting amount of C-peptide bound to said at least one anti-C-peptide antibody in said contacted sample as an assay of the amount of C-peptide in the sample. |
| Claim: |
27. A hybridoma deposited at Collection Nationale de Cultures de Microorganismes at the Institut Pasteur on 4 Jun. 1997, registration number I-1873. |
| Current U.S. Class: |
435007/100 |
| رقم الانضمام: |
edspap.20050164303 |
| قاعدة البيانات: |
USPTO Patent Applications |