Academic Journal
Single-Molecule Investigation of the Binding Interface Stability of SARS-CoV-2 Variants with ACE2.
| العنوان: | Single-Molecule Investigation of the Binding Interface Stability of SARS-CoV-2 Variants with ACE2. |
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| المؤلفون: | Ray, Ankita, Minh Tran, Thu Thi, Santos Natividade, Rita Dos, Moreira, Rodrigo A, Simpson, Joshua D, Mohammed, Danahe, Koehler, Melanie, L Petitjean, Simon J, Zhang, Qingrong, Bureau, Fabrice, Gillet, Laurent, Poma, Adolfo B, Alsteens, David |
| المصدر: | ACS Nanoscience Au, 4 (2), 136 - 145 (2024-04-17) |
| بيانات النشر: | American Chemical Society, 2024. |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | ACE2, Atomic force microscopy, biolayer interferometry, convalescent patient sera, RBD, SARS-CoV-2, Steered molecular dynamics, Chemistry (miscellaneous), Materials Science (miscellaneous), Human health sciences, Immunology & infectious disease, Sciences de la santé humaine, Immunologie & maladie infectieuse |
| الوصف: | The SARS-CoV-2 pandemic spurred numerous research endeavors to comprehend the virus and mitigate its global severity. Understanding the binding interface between the virus and human receptors is pivotal to these efforts and paramount to curbing infection and transmission. Here we employ atomic force microscopy and steered molecular dynamics simulation to explore SARS-CoV-2 receptor binding domain (RBD) variants and angiotensin-converting enzyme 2 (ACE2), examining the impact of mutations at key residues upon binding affinity. Our results show that the Omicron and Delta variants possess strengthened binding affinity in comparison to the Mu variant. Further, using sera from individuals either vaccinated or with acquired immunity following Delta strain infection, we assess the impact of immunity upon variant RBD/ACE2 complex formation. Single-molecule force spectroscopy analysis suggests that vaccination before infection may provide stronger protection across variants. These results underscore the need to monitor antigenic changes in order to continue developing innovative and effective SARS-CoV-2 abrogation strategies. |
| نوع الوثيقة: | journal article http://purl.org/coar/resource_type/c_6501 article peer reviewed |
| اللغة: | English |
| Relation: | https://pubs.acs.org/doi/pdf/10.1021/acsnanoscienceau.3c00060; urn:issn:2694-2496 |
| DOI: | 10.1021/acsnanoscienceau.3c00060 |
| URL الوصول: | https://orbi.uliege.be/handle/2268/320928 |
| Rights: | open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsorb.320928 |
| قاعدة البيانات: | ORBi |
| DOI: | 10.1021/acsnanoscienceau.3c00060 |
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