Conference
NanoLuX: a network-wide Nanoluciferase-based platform to monitor activation of classical and atypical chemokine receptors
| العنوان: | NanoLuX: a network-wide Nanoluciferase-based platform to monitor activation of classical and atypical chemokine receptors |
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| المؤلفون: | Meyrath, Max Marc Roger, Reynders, Nathan, Counson, Manuel, Beaupain, Nadia, Ollert, Markus, Szpakowska, Martyna, Chevigne, Andy |
| المصدر: | 3rd European Chemokine and Cell Migration Conference (ECMC2019), Salamanca, Spain [ES], 26-06-2019 to 30-06-2019 |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | chemokine receptors, Nanoluciferase, arrestin, Life sciences, Biochemistry, biophysics & molecular biology, Sciences du vivant, Biochimie, biophysique & biologie moléculaire |
| الوصف: | The activity of chemokine receptors is dependent on G proteins that, upon chemokinebinding, trigger various intracellular signalling cascades, as well as on b-arrestins that,following receptor phosphorylation by GRK, orchestrate its desensitization,endocytosis and trafficking. In addition to the 19 classical chemokine receptors, 4receptors form a subfamily of atypical chemokine receptors (ACKR1-4) with ligand scavengingfunctions (Fig 1). These receptors are unable to couple to G proteins buttheir activity can be monitored via b-arrestins.In this study we describe the NanoLux platform, a network-wide profiling platform forchemokines and chemokines receptors based on the complementation of thenanoluciferase (NanoBiT). This platform allows to monitor the activation, modulationor bias of receptors or ligands by measuring the binding or the recruitment ofeffectors, regulators or parners such as G proteins, GRK or b-arrestin isoforms to theclassical and the atypical chemokine receptors. For that purpose, we N-terminallyfused the LgBiT portion of Nanoluciferase to the MiniGi protein, GRK2 or human b-arrestin 1 and 2, while the SmBiT was fused to the C terminus of all of the 23 humanchemokine receptors (Fig 2). Using this approach, we are now able to assess andcompare the activity of molecules at the chemokine-receptor network level. |
| نوع الوثيقة: | conference poster not in proceedings http://purl.org/coar/resource_type/c_18co conferencePoster |
| وصف الملف: | A0 |
| اللغة: | English |
| URL الوصول: | https://orbi.uliege.be/handle/2268/238132 |
| Rights: | restricted access http://purl.org/coar/access_right/c_16ec info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsorb.238132 |
| قاعدة البيانات: | ORBi |
| الوصف غير متاح. |