Electronic Resource
Cholesterol accumulation in ovarian follicles causes ovulation defects in Abca1a⁻/⁻ Japanese medaka (Oryzias latipes)
| العنوان: | Cholesterol accumulation in ovarian follicles causes ovulation defects in Abca1a⁻/⁻ Japanese medaka (Oryzias latipes) |
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| المؤلفون: | 60263125, 90234179, 10151789, Futamata, Ryota, Kinoshita, Masato, Ogiwara, Katsueki, Kioka, Noriyuki, Ueda, Kazumitsu |
| بيانات النشر: | Elsevier BV 2023-02 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | ATP-binding cassette A1 (ABCA1) is a membrane protein, which exports excess cellular cholesterol to generate HDL to reduce the risk of the onset of cardiovascular diseases (CVD). In addition, ABCA1 exerts pleiotropic effects on such as inflammation, tissue repair, and cell proliferation and migration. In this study, we explored the novel physiological roles of ABCA1 using Japanese medaka (Oryzias latipes), a small teleost fish. Three Abca1 genes were found in the medaka genome. ABCA1A and ABCA1C exported cholesterol to generate nascent HDL as human ABCA1 when expressed in HEK293 cells. To investigate their physiological roles, each Abca1-deficient fish was generated using the CRISPR-Cas9 system. Abca1a−/− female medaka was found to be infertile, while Abca1b−/− and Abca1c−/− female medaka were fertile. In vitro ovarian follicle culture suggested that Abca1a deficiency causes ovulation defects. In the ovary, ABCA1A was expressed in theca cells, an outermost layer of the ovarian follicle. Total cholesterol content of Abca1a−/− ovary was significantly higher than that of the wild-type, while estrogen and progestin contents were compatible with those of the wild-type. Furthermore, cholesterol loading to the wild-type follicles caused ovulation defects. These results suggest that ABCA1A in theca cells regulates cholesterol content in the ovarian follicles and its deficiency inhibits successful ovulation through cholesterol accumulation in the ovarian follicle. |
| مصطلحات الفهرس: | Abca1a−/− medaka causes ovulation defects, ABC transporter, ABCA1, Japanese medaka, Cholesterol, Ovary, Ovulation, journal article |
| URL: | 10.1016/j.heliyon.2023.e13291 |
| الاتاحة: | Open access content. Open access content http://creativecommons.org/licenses/by/4.0 © 2023 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license. |
| ملاحظة: | English |
| Other Numbers: | YJ@ oai:repository.kulib.kyoto-u.ac.jp:2433/282111 Heliyon 9 2 e13291 36816300 2405-8440 2405-8440 1458646448 |
| المصدر المساهم: | KYOTO UNIV From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1458646448 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |