Electronic Resource
Uncovering strain- and age-dependent innate immune responses to SARS-CoV-2 infection in air-liquid-interface cultured nasal epithelia
| العنوان: | Uncovering strain- and age-dependent innate immune responses to SARS-CoV-2 infection in air-liquid-interface cultured nasal epithelia |
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| المؤلفون: | Chang, JJ-Y, Grimley, SL, Tran, BM, Deliyannis, G, Tumpach, C, Nguyen, ANT, Steinig, E, Zhang, J, Schroder, J, Caly, L, McAuley, J, Wong, SL, Waters, SA, Stinear, TP, Pitt, ME, Purcell, D, Vincan, E, Coin, LJM |
| بيانات النشر: | CELL PRESS 2024-06-21 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Continuous assessment of the impact of SARS-CoV-2 on the host at the cell-type level is crucial for understanding key mechanisms involved in host defense responses to viral infection. We investigated host response to ancestral-strain and Alpha-variant SARS-CoV-2 infections within air-liquid-interface human nasal epithelial cells from younger adults (26-32 Y) and older children (12-14 Y) using single-cell RNA-sequencing. Ciliated and secretory-ciliated cells formed the majority of highly infected cell-types, with the latter derived from ciliated lineages. Strong innate immune responses were observed across lowly infected and uninfected bystander cells and heightened in Alpha-infection. Alpha highly infected cells showed increased expression of protein-refolding genes compared with ancestral-strain-infected cells in children. Furthermore, oxidative phosphorylation-related genes were down-regulated in bystander cells versus infected and mock-control cells, underscoring the importance of these biological functions for viral replication. Overall, this study highlights the complexity of cell-type-, age- and viral strain-dependent host epithelial responses to SARS-CoV-2. |
| مصطلحات الفهرس: | Journal Article |
| URL: | NHMRC/1181580 |
| الاتاحة: | Open access content. Open access content CC BY https://creativecommons.org/licenses/by/4.0 |
| Other Numbers: | UMV oai:jupiter.its.unimelb.edu.au:11343/349028 Chang, J. J. -Y., Grimley, S. L., Tran, B. M., Deliyannis, G., Tumpach, C., Nguyen, A. N. T., Steinig, E., Zhang, J., Schroder, J., Caly, L., McAuley, J., Wong, S. L., Waters, S. A., Stinear, T. P., Pitt, M. E., Purcell, D., Vincan, E. & Coin, L. J. M. (2024). Uncovering strain- and age-dependent innate immune responses to SARS-CoV-2 infection in air-liquid-interface cultured nasal epithelia. ISCIENCE, 27 (6), https://doi.org/10.1016/j.isci.2024.110009. 10.1016/j.isci.2024.110009 2589-0042 2589-0042 1456027076 |
| المصدر المساهم: | UNIV OF MELBOURNE From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1456027076 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |