Electronic Resource
Selective tumor antigen vaccine delivery to human CD169+ antigen-presenting cells using ganglioside-liposomes
| العنوان: | Selective tumor antigen vaccine delivery to human CD169+ antigen-presenting cells using ganglioside-liposomes |
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| المؤلفون: | Affandi, Alsya J, Grabowska, Joanna, Olesek, Katarzyna, Lopez Venegas, Miguel, Barbaria, Arnaud, Rodríguez, Ernesto, Mulder, Patrick P G, Pijffers, Helen J, Ambrosini, Martino, Kalay, Hakan, O'Toole, Tom, Zwart, Eline S, Kazemier, Geert, Nazmi, Kamran, Bikker, Floris J, Stöckl, Johannes, van den Eertwegh, Alfons J M, de Gruijl, Tanja D, Storm, Gert, van Kooyk, Yvette, den Haan, Joke M M |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America vol.117 (2020) date: 2020-11-02 nr.44 p.27528-27539 [ISSN 0027-8424] |
| بيانات النشر: | 2020 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Priming of CD8+ T cells by dendritic cells (DCs) is crucial for the generation of effective antitumor immune responses. Here, we describe a liposomal vaccine carrier that delivers tumor antigens to human CD169/Siglec-1+ antigen-presenting cells using gangliosides as targeting ligands. Ganglioside-liposomes specifically bound to CD169 and were internalized by in vitro-generated monocyte-derived DCs (moDCs) and macrophages and by ex vivo-isolated splenic macrophages in a CD169-dependent manner. In blood, high-dimensional reduction analysis revealed that ganglioside-liposomes specifically targeted CD14+ CD169+ monocytes and Axl+ CD169+ DCs. Liposomal codelivery of tumor antigen and Toll-like receptor ligand to CD169+ moDCs and Axl+ CD169+ DCs led to cytokine production and robust cross-presentation and activation of tumor antigen-specific CD8+ T cells. Finally, Axl+ CD169+ DCs were present in cancer patients and efficiently captured ganglioside-liposomes. Our findings demonstrate a nanovaccine platform targeting CD169+ DCs to drive antitumor T cell responses. |
| مصطلحات الفهرس: | Siglec-1, CD169, dendritic cells, CD8+ T cells, vaccination, Gangliosides, Humans, Immunogenicity, Vaccine, Leukocytes, Mononuclear, Liposomes, Macrophages/immunology, Neoplasms/immunology, Primary Cell Culture, Proto-Oncogene Proteins/metabolism, Receptor Protein-Tyrosine Kinases/metabolism, Sialic Acid Binding Ig-like Lectin 1/metabolism, THP-1 Cells, Vaccination/methods, Vaccines, Subunit/administration & dosage, Article |
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| الاتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | DOI: 10.1073/pnas.2006186117 English |
| Other Numbers: | QGJ oai:research-portal.uu.nl:publications/c76b00a3-3485-4423-801b-ece26acc545f 1445817739 |
| المصدر المساهم: | UNIVERSITEIT UTRECHT From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1445817739 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |