Epithelial-to-mesenchymal transition in fibrosis: collagen type I expression is highly upregulated after EMT, but does not contribute to collagen deposition.
| العنوان: | Epithelial-to-mesenchymal transition in fibrosis: collagen type I expression is highly upregulated after EMT, but does not contribute to collagen deposition. |
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| المؤلفون: | Hosper, N.A. |
| Added Details: | Berg, P.P. van den author. Rond, S. de author. Popa, E.R. author. Wilmer, M.J.G. author. Masereeuw, R. author. Bank, RA author. |
| وصف مادي: | 1 online resource |
| الموضوعات: | IGMD 9: Renal disorder., NCMLS 5: Membrane transport and intracellular motility IGMD 9: Renal disorder. |
| مصطلحات الفهرس: | Article / Letter to editor. |
| URL: | |
| الاتاحة: | Open access content. Open access content. |
| ملاحظة: | Experimental Cell Research, 319, 19, pp. 3000-9. Contains fulltext : 125420.pdf (Publisher's version ) (Closed access). The hallmark of fibrosis is an accumulation of fibrillar collagens, especially of collagen type I. There is considerable debate whether in vivo type II epithelial-to-mesenchymal transition (EMT) is involved in organ fibrosis. Lineage tracing experiments by various groups show opposing data concerning the relative contribution of epithelial cells to the pool of myofibroblasts. We hypothesized that EMT-derived cells might directly contribute to collagen deposition. To study this, EMT was induced in human epithelial lung and renal cell lines in vitro by means of TGF-beta1 stimulation, and we compared the collagen type I (COL1A1) expression levels of transdifferentiated cells with that of myofibroblasts obtained by TGF-beta1 stimulation of human dermal and lung fibroblasts. COL1A1 expression levels of transdifferentiated epithelial cells appeared to be at least one to two orders of magnitude lower than that of myofibroblasts. This was confirmed at immunohistochemical level: in contrast to myofibroblasts, collagen type I deposition by EMT-derived cells was not or hardly detectable. We postulate that, even when type II EMT occurs in vivo, the direct contribution of EMT-derived cells to collagen accumulation is rather limited. |
| Other Numbers: | NLQGE oai:repository.ubn.ru.nl:2066/125420 1443214877 |
| المصدر المساهم: | From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1443214877 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |