Electronic Resource
Experimental Analysis of Lifelines in a 15,000 L Bioreactor by Means of Lagrangian Sensor Particles
| العنوان: | Experimental Analysis of Lifelines in a 15,000 L Bioreactor by Means of Lagrangian Sensor Particles |
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| المؤلفون: | (0000-0002-4312-7402) Hofmann, S., (0000-0003-3721-489X) Buntkiel, L., (0000-0002-6943-2077) Rautenbach, R., (0000-0001-6817-9068) Gaugler, L., (0009-0003-8990-665X) Ma, Y., Haase, I., (0000-0002-1418-933X) Fitschen, J., Wucherpfennig, T., (0000-0003-2705-0692) Reinecke, S., Hoffmann, M., Takors, R., (0000-0002-7371-0148) Hampel, U., (0000-0001-5969-2150) Schlüter, M. |
| المصدر: | Chemical Engineering Research and Design 205(2024), 0263-8762 |
| بيانات النشر: | 2024 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | This study employs Lagrangian Sensor Particles (LSPs) with a diameter of 40 mm equipped with a pressure sensor to investigate cell lifelines in a 15,000 L stirred tank reactor (STR) with three Elephant Ear impellers. The Stokes number of the LSPs is approx. 0.06 on a macro-scale. The vertical probability of presence, axial velocity profiles, circulation time distributions, and residence time distributions are quantified to analyze single-phase mixing heterogeneities, detect hydrodynamic compartments and conduct a Lagrangian regime analysis. Results reveal a similarly distributed probability of presence in the vertical reactor center but emphasize the LSP's sensitivity to fluctuating densities. Axial velocity distributions illustrate characteristic impeller-induced flow patterns, and circulation time distributions identify three compartments with comparatively shorter times in the axial center. Residence time distributions exhibit a similar compartmentalized profile. Moreover, the study estimates a potential oxygen deprivation zone for CHO cells in the upper compartment and demonstrates the LSP's efficacy in characterizing impeller systems. Contrary to literature, the ratio of examined global mixing times to circulation times is 1.0, highlighting macro-scale mixing. The research underscores that LSPs offer crucial insights into industrial-scale STRs, specifically for determining hydrodynamic compartments without having optical access. |
| مصطلحات الفهرس: | Lagrangian Sensor Particles, Circulation Time Distribution, Residence Time Distribution, Lagrangian Regime Analysis, Elephant Ear Impeller, Industrial Scale, info:eu-repo/semantics/publishedVersion, doc-type:article, Text |
| URL: | info:eu-repo/semantics/reference/doi/10.1016/j.cherd.2024.04.015 info:eu-repo/semantics/altIdentifier/purl/https://www.hzdr.de/publications/Publ-38091-1 |
| الاتاحة: | Open access content. Open access content Open Access |
| ملاحظة: | application/pdf English |
| Other Numbers: | DERBS HZDR:PUBLDB:38091-1 1440068201 |
| المصدر المساهم: | ROBIS REPOSITORY From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1440068201 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |