Electronic Resource
Microwave contactless current-sensing for live/dead differentiation of single bioparticles on a microfluidic platform
| العنوان: | Microwave contactless current-sensing for live/dead differentiation of single bioparticles on a microfluidic platform |
|---|---|
| Additional Titles: | Detección de corriente sin contacto de microondas para diferenciación en vivo/muerto de biopartículas individuales en una plataforma microfluidic |
| المؤلفون: | Universitat Politècnica de Catalunya. Doctorat en Teoria del Senyal i Comunicacions, Universitat Politècnica de Catalunya. Departament d'Enginyeria Telemàtica, Universitat Politècnica de Catalunya. Departament de Mecànica de Fluids, Universitat Politècnica de Catalunya. Departament de Teoria del Senyal i Comunicacions, Universitat Politècnica de Catalunya. GReCEF- Grup de Recerca en Ciència i Enginyeria de Fluids, Universitat Politècnica de Catalunya. CommSensLab-UPC - Centre Específic de Recerca en Comunicació i Detecció UPC, Palacios Arias, César Augusto, Jofre Cruanyes, Marc, Jofre Cruanyes, Lluís, Romeu Robert, Jordi, Jofre Roca, Lluís |
| بيانات النشر: | Institute of Electrical and Electronics Engineers (IEEE) 2023-08-02 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | © 2023 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works. The combination of microwave and microfluidic technologies enables the creation of efficient platforms to rapidly quantify live/dead bioparticles in industrial processes, biomedical research and environmental monitoring applications. In this regard, this work demonstrates the differentiation of single live/dead bioparticles with a microwave-microfluidic platform. The system is composed of a contactless current-sensing superheterodyne architecture with broadband frequency-operation bowtie electrodes. Furthermore, the 20mm × 20mm bowtie electrode has been designed and manufactured with a –10 dB frequency range, from 4GHz to 8GHz, and capable of confining the electric field in a micrometric volume. The gap of the bowtie geometry of the electrodes is adjusted to the dimensions of the 50 µm × 50 µm cross-section and 40mm long microfluidic channel. In particular, 10mL sample with 10 9 bacteria/mL flows through the microchannel at 19.8 µL/min, focused with elasto-inertial effect enhanced with 500 ppm of polyethylene oxide. Accordingly, from the signal captured by the electrodes, the energy variation of the applied electric field produced by the bioparticles is sensed by a current-based transimpedance amplifier. Here, the incorporation of the transimpedance amplifier in the measurement system improves the SNR by 4 dB. In addition, the superheterodyne transceiver has been optimized in terms of local oscillator power and bandwidth. Furthermore, the microfluidic system has been adjusted so that the balance of hydrodynamic forces confines bioparticles at the centerline of the microchannel within less than 5 µm. As a result, the system measures the cytoplasmic content of live and dead flowing bacteria with a difference of 6 dB and relative deviation of 3.2%. This work was financially supported by the PID2019- 107885GB-C31 and PDC2022-133091-I00 grants of the Agencia Estatal de Investigación (Spain), and the PDR-2014- 2022/56-30157-2021-2A grant from Generalitat de Catalunya (Spain). In addition, the authors are grateful for the support provided by the Formación de Personal Investigador (PID2019-107885GB-C31, PRE2020-093895) scholarship and the Beatriz Galindo (BGP18/00026) program of the Ministerio de Ciencia, Innovación y Universidades (Spain). Peer Reviewed Postprint (author's final draft) |
| مصطلحات الفهرس: | Àrees temàtiques de la UPC::Enginyeria de la telecomunicació, Microfluidics, Microwave circuits, Contactless, Electrodes, Microwaves, Sensing, Single-cell detection, Superheterodyne, System-On-a-Chip, Microfluídica, Circuits de microones, Article |
| URL: | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107885GB-C31/ES/SISTEMAS RADIANTES X-WAVE INTEGRADAS DE COMUNICACIONES Y SENSORIZACION info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PDC2022-133091-I00/ES/MONITORIZACION DE LA ACTIVIDAD CELULAR MEDIANTE RF |
| الاتاحة: | Open access content. Open access content Restricted access - publisher's policy |
| ملاحظة: | application/pdf English |
| Other Numbers: | HGF oai:upcommons.upc.edu:2117/406154 Palacios, C. [et al.]. Microwave contactless current-sensing for live/dead differentiation of single bioparticles on a microfluidic platform. "IEEE transactions on instrumentation and measurement", 2 Agost 2023, vol. 72, núm. article 8004310. 1557-9662 10.1109/TIM.2023.3280521 1439653802 |
| المصدر المساهم: | UNIV POLITECNICA DE CATALUNYA From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1439653802 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |