Electronic Resource
Pathology Laboratory Archives: Conservation Quality of Nucleic Acids and Proteins for NSCLC Molecular Testing
| العنوان: | Pathology Laboratory Archives: Conservation Quality of Nucleic Acids and Proteins for NSCLC Molecular Testing |
|---|---|
| المؤلفون: | Eccher, A, Seminati, D, L’Imperio, V, Casati, G, Pilla, D, Malapelle, U, Piga, I, Bindi, G, Marando, A, Bonoldi, E, Dainese, E, Riefolo, M, D’Errico, A, Costantini, M, Lugli, A, Grassi, S, Scarpa, A, Dei Tos, A, Pagni, F, Eccher, Albino, Seminati, Davide, L’Imperio, Vincenzo, Casati, Gabriele, Pilla, Daniela, Malapelle, Umberto, Piga, Isabella, Bindi, Greta, Marando, Alessandro, Bonoldi, Emanuela, Dainese, Emanuele, Riefolo, Mattia, D’Errico, Antonia, Costantini, Matteo, Lugli, Alberto, Grassi, Stefano, Scarpa, Aldo, Dei Tos, Angelo Paolo, Pagni, Fabio |
| بيانات النشر: | MDPI country:CH 2024 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | In the molecular era, proper archival conditions within pathology laboratories are crucial, especially for formalin-fixed paraffin-embedded (FFPE) tissue specimens retrieved years after the original diagnosis. Indeed, improper preservation can impact the integrity of nucleic acids and protein antigens. This study evaluates the quality status of stored FFPE blocks using multilevel omics approaches. FFPE blocks from 45 Non-Small Cell Lung Carcinoma (NSCLC) cases were analyzed. The blocks were collected from six different pathology archives across Italy with distinct environmental characteristics. Nucleic acids’ quantity and quality, as well as protein antigens, were assessed using various techniques, including MALDI-MSI. RNA was quantitatively higher, but more fragmented, compared to DNA. DNA quantity and quality were suitable for molecular analyses in 94.4% and 62.3% of samples, respectively. RNA quantity was adequate across all samples, but it was optimal only in 22.3% of cases. DNA quality started to deteriorate after 6–8 years, whereas RNA quality diminished only after 10 years of storage. These data might suggest a particular DNA susceptibility to FFPE blocks conservation. Immunohistochemical intensity decreased significantly after 6–8 years of storage, and MALDI-MSI analysis revealed that younger tissue blocks contained more unique proteomic signals than the older ones. This study emphasizes the importance of proper FFPE archiving conditions for molecular analyses. Governance should prioritize attention to pathology archives to ensure quality preservation and optimize predictive testing. By elucidating the nuances of FFPE block storage, this research paves the way for enhanced molecular diagnostics and therapeutic insights regarding oncology and beyond. |
| مصطلحات الفهرس: | conservation quality, FFPE block, NSCLC, pathology laboratory archive, info:eu-repo/semantics/article |
| URL: | info:eu-repo/semantics/altIdentifier/pmid/38672960 info:eu-repo/semantics/altIdentifier/wos/WOS:001210417000001 volume:14 issue:4 journal:JOURNAL OF PERSONALIZED MEDICINE |
| الاتاحة: | Open access content. Open access content |
| ملاحظة: | ELETTRONICO English |
| Other Numbers: | ITBAO oai:boa.unimib.it:10281/474420 10.3390/jpm14040333 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85191761491 1434547451 |
| المصدر المساهم: | BICOCCA OPEN ARCH From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1434547451 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |