Electronic Resource
Autophagy is activated and involved in cell death with participation of cathepsins during stress-induced microspore embryogenesis in barley
| العنوان: | Autophagy is activated and involved in cell death with participation of cathepsins during stress-induced microspore embryogenesis in barley |
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| المؤلفون: | Bárány, Ivett, Berenguer, Eduardo, Solís González, María Teresa, Pérez Pérez, Yolanda, Santamaría, M. Estrella, Crespo, José Luis, Risueño, María C., Díaz, Isabel, Testillano, Pilar S. |
| بيانات النشر: | Oxford University Press 2024-01-30T17:59:48Z 2024-01-30T17:59:48Z 2018-03-05 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | This work is supported by projects AGL2014-52028-R and AGL2017-82447-R funded by the Spanish Ministry of Economy and Competitiveness (MINECO) and the European Regional Development Fund (ERDF/FEDER). YPP is the recipient of a grant (PEJ15/BIO/AI-01S8) funded by Comunidad de Madrid and European Commission through ERDF/FEDER. Microspores are reprogrammed towards embryogenesis by stress. Many microspores die after this stress, limiting the efficiency of microspore embryogenesis. Autophagy is a degradation pathway that plays critical roles in stress response and cell death. In animals, cathepsins have an integral role in autophagy by degrading autophagic material; less is known in plants. Plant cathepsins are papain-like C1A cysteine proteases involved in many physiological processes, including programmed cell death. We have analysed the involvement of autophagy in cell death, in relation to cathepsin activation, during stress-induced microspore embryogenesis in Hordeum vulgare. After stress, reactive oxygen species (ROS) and cell death increased and autophagy was activated, including HvATG5 and HvATG6 up-regulation and increase of ATG5, ATG8, and autophagosomes. Concomitantly, cathepsin L/F-, B-, and H-like activities were induced, cathepsin-like genes HvPap-1 and HvPap-6 were up-regulated, and HvPap-1, HvPap-6, and HvPap-19 proteins increased and localized in the cytoplasm, resembling autophagy structures. Inhibitors of autophagy and cysteine proteases reduced cell death and promoted embryogenesis. The findings reveal a role for autophagy in stress-induced cell death during microspore embryogenesis, and the participation of cathepsins. Similar patterns of activation, expression, and localization suggest a possible connection between cathepsins and autophagy. The results open up new possibilities to enhance microspore embryogenesis efficiency with autophagy and/or cysteine protease modulators. Depto. de Genética, Fisiología y Microbiología Fac. de Ciencias Biológicas TRUE pub |
| مصطلحات الفهرس: | 576.32, Autophagy, Barley, Caspase-like activity, Cathepsins, Cell death, Cysteine C1A proteases, Microspore embryogenesis, ROS, Stress response, Biología celular (Biología), Biotecnología, 2407 Biología Celular, journal article, VoR |
| URL: | |
| الاتاحة: | Open access content. Open access content Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 open access |
| ملاحظة: | application/pdf 0022-0957 English |
| Other Numbers: | ESRCM oai:docta.ucm.es:20.500.14352/96741 Bárány I, Berenguer E, Solís MT, Pérez-Pérez Y, Santamaría ME, Crespo JL, Risueño MC, Díaz I, Testillano PS. Autophagy is activated and involved in cell death with participation of cathepsins during stress-induced microspore embryogenesis in barley. J Exp Bot. 2018 Mar 14;69(6):1387-1402. doi: 10.1093/jxb/erx455. PMID: 29309624; PMCID: PMC6019037. 0022-0957 10.1093/JXB/ERX455 1460-2431 1429624441 |
| المصدر المساهم: | REPOSITORIO E-PRINTS UNIVERSIDAD COMPLU From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1429624441 |
| قاعدة البيانات: | OAIster |
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