Electronic Resource
Generation of the Y-chromosome linked red fluorescent protein transgenic mouse model and sexing at the preimplantation stage
| العنوان: | Generation of the Y-chromosome linked red fluorescent protein transgenic mouse model and sexing at the preimplantation stage |
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| المؤلفون: | Hirata, Wataru, Tomoda, Taiki, 10800881, Yuri, Shunsuke, 20444523, Isotani, Ayako |
| بيانات النشر: | Japanese Association for Laboratory Animal Science 2022-02-09 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | In mammals, sexual fate is determined by the chromosomes of the male and female gametes during fertilization. Males (XY) or females (XX) are produced when a sperm containing a Y or X-chromosome respectively fertilizes an X-chromosome-containing unfertilized egg. However, sexing of preimplantation stage embryos cannot be conducted visually. To address this, transgenic male mouse models with the ubiquitously expressed green fluorescent protein (GFP) transgene on X- (X-GFP) or Y-chromosomes (Y-GFP) have been established. However, when crossed with wild-type females, sexing of the preimplantation stage embryos by observing the GFP signal is problematic in some cases due to X-inactivation, loss of Y-chromosome (LOY), or loss of transgene fluorescence. In this study, a mouse model with the ubiquitously expressed red fluorescent protein (RFP) transgene on the Y-chromosome was generated since RFP is easily distinguishable from GFP signals. Unfortunately, the ubiquitously expressed tdTomato RFP transgene on the Y-chromosome (Y-RFP) mouse showed the lethal phenotype after birth. No lethal phenotypes were observed when the mitochondrial locating signal N-terminal of tdTomato (mtRFP) was included in the transgene construct. Almost half of the collected fertilized eggs from Y-mtRFP male mice crossed with wild-type females had an RFP signal at the preimplantation stage (E1.5). Therefore, XY eggs were recognized as RFP-positive embryos at the preimplantation stage. Furthermore, 100% sexing was observed at the preimplantation stage using the X-linked GFP/Y-linked RFP male mouse. The established Y-mtRFP mouse models may be used to study sex chromosome related research. |
| مصطلحات الفهرس: | preimplantation embryo, sex chromosome, sexing, tdTomato, Journal Article |
| URL: | 34544911 |
| الاتاحة: | Open access content. Open access content metadata only access ©2022 Japanese Association for Laboratory Animal Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License <http://creativecommons.org/licenses/by-nc-nd/4.0/>. |
| ملاحظة: | English |
| Other Numbers: | JPNII oai:irdb.nii.ac.jp:01146:0005787866 1881-7122 Experimental Animals, 71(1), 82-89 1409772434 |
| المصدر المساهم: | NATIONAL INST OF INFO From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1409772434 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |