Electronic Resource

Hereditary Pancreatitis Model by Blastocyst Complementation in Mouse

التفاصيل البيبلوغرافية
العنوان: Hereditary Pancreatitis Model by Blastocyst Complementation in Mouse
المؤلفون: Asai, Ayumu, Konno, Masamitsu, Kawamoto, Koichi, 20444523, Isotani, Ayako, Mori, Masaki, Eguchi, Hidetoshi, Doki, Yuichiro, Arai, Takahiro, Ishii, Hideshi
بيانات النشر: Impact Journals 2020-06-02
نوع الوثيقة: Electronic Resource
مستخلص: The application of pluripotent stem cells is expected to contribute to the elucidation of unknown mechanism of human diseases. However, in vitro induction of organ-specific cells, such as pancreas and liver, is still difficult and the reproduction of their disorders in a model has been unfeasible. To study the mechanism of human hereditary pancreatitis (HP), we here performed the blastocyst complementation (BC) method. In the BC method, mouse embryonic stem (ES) cells harboring CRISPR/CAS9-mediated mutations in the Prss1 gene were injected into blastocysts with deficient Pdx1 gene, which is a critical transcription factor in the development of pancreas. The results showed that trypsin was activated extremely in Prss1-mutant mice. This implied that the mouse phenotype mimics that of human HP and that the BC method was useful for the reproduction and study of pancreatic disorders. The present study opens the possibility of investigating uncharacterized human diseases by utilizing the BC method.
مصطلحات الفهرس: blastocyst complementation, hereditary pancreatitis, PRSS1, Journal Article
URL: http://hdl.handle.net/10061/14029
https://naist.repo.nii.ac.jp/records/9261
https://doi.org/10.18632/oncotarget.27595
https://www.oncotarget.com/article/27595/text/
32547704
https://doi.org/10.18632/oncotarget.27595
https://www.oncotarget.com/article/27595/text
الاتاحة: Open access content. Open access content
metadata only access
Asai et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and sourceare credited.
ملاحظة: English
Other Numbers: JPNII oai:irdb.nii.ac.jp:01146:0005787712
1949-2553
Oncotarget, 11(22), 2061-2073
1409771788
المصدر المساهم: NATIONAL INST OF INFO
From OAIster®, provided by the OCLC Cooperative.
رقم الانضمام: edsoai.on1409771788
قاعدة البيانات: OAIster