Electronic Resource
In vitro investigation of gastrointestinal stability and toxicity of 3-hyrdoxypropionaldehyde (reuterin) produced by Lactobacillus reuteri
| العنوان: | In vitro investigation of gastrointestinal stability and toxicity of 3-hyrdoxypropionaldehyde (reuterin) produced by Lactobacillus reuteri |
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| المؤلفون: | Soltani, Samira, Couture, Frédéric, Boutin, Yvan., Ben Said, Laila, Cashman-Kadri, Samuel, Subirade, Muriel, Biron, Éric, Fliss, Ismaïl |
| بيانات النشر: | Elsevier 2023-09-08T15:31:52Z 2023-09-08T15:31:52Z 2021-03-31 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Reuterin (3-hyrdoxypropionaldehyde (3-HPA)) is a highly potent metabolite of L. reuteri, which has applications in food, health, and veterinary sectors. Similar to other natural antimicrobial compounds, the approval of reuterin as a bio-preservative or therapeutic agent by regulatory agencies relies on sufficient data on its cytotoxicity and behavior in the gastrointestinal environment. Although the antimicrobial activity of reuterin has been broadly studied, its safety and toxicity are yet to be explored in detail. In this study, the stability and activity of reuterin were investigated in the gastrointestinal tract using in vitro models simulating gastrointestinal conditions. In addition, hemolytic activity and in vitro cytotoxicity of reuterin were evaluated by neutral red assay and lactate dehydrogenase (LDH) colorimetric assay using the same cell line. Activity of reuterin was observed to be stable during gastrointestinal transit. Viability and membrane integrity of cells remained unaltered by reuterin up to 1080 mM concentration. Furthermore, no hemolysis was observed in blood cells exposed to 270 mM reuterin. This study provides unique and highly relevant in vitro data regarding gastrointestinal behavior and toxicity of reuterin. In conclusion, the current study indicates that within a certain concentration range, reuterin can be safely used in bio-preservation and therapeutics applications. However, further in vivo studies are required to confirm these findings. |
| مصطلحات الفهرس: | Reuterin, Antimicrobial activity, Cytotoxicity, In vitro digestion, Eukaryotic cells, Lactobacillus, Métabolites, Antiinfectieux, Digestion, Tractus gastro-intestinal, Toxicité in vitro, article de recherche |
| URL: | |
| الاتاحة: | Open access content. Open access content http://purl.org/coar/access_right/c_abf2 |
| ملاحظة: | application/pdf English |
| Other Numbers: | LVB oai:corpus.ulaval.ca:20.500.11794/124763 2214-7500 10.1016/j.toxrep.2021.03.025 33868958 1409180034 |
| المصدر المساهم: | UNIVERSITE LAVAL From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1409180034 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |